| 活性污泥样品荧光原位杂交实验方法研究 |
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| 引用本文： | 聂英进,孙晓莹,张轶凡,林姣.活性污泥样品荧光原位杂交实验方法研究[J].四川环境,2011(4):5-8. |
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| 作者姓名： | 聂英进 孙晓莹 张轶凡 林姣 |
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| 作者单位： | 天津创业环保集团股份有限公司;中国市政工程华北设计研究总院; |
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| 基金项目： | 水体污染控制与治理科技重大专项(2009ZX07314-002) |
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| 摘 要： | 采用细菌通用探针EUB338,结合DAPI全细胞染色技术,探讨了针对活性污泥样品的荧光原位杂交实验方法,通过调整样品预处理方法及固定剂类型得到了较理想的荧光原位杂交图片,可以清晰的辨别被杂交细菌的大小、形态及分布,但与探针EUB338杂交的细胞和被DAPI染色的细胞尚不能完全重合,因此仍需进一步改进实验方法。
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| 关 键 词： | 活性污泥 固定 杂交 自发荧光 |
Methodology of Experiment on Fluorescence in Situ Hybridization Experiment for Active Sludge |
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| NIE Ying-jin,SUN Xiao-ying,ZHANG Yi-fan,LIN Jiao.Methodology of Experiment on Fluorescence in Situ Hybridization Experiment for Active Sludge[J].Sichuan Environment,2011(4):5-8. |
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| Authors: | NIE Ying-jin SUN Xiao-ying ZHANG Yi-fan LIN Jiao |
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| Institution: | NIE Ying-jin1,SUN Xiao-ying1,ZHANG Yi-fan1,LIN Jiao2(1.Tianjin Capital Environmental Protection Company Limited,Tianjin 300381,China,2.North China Municipal Engineering Design & Research Institute,Tianjin 300074,China) |
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| Abstract: | Bacterial universal probe EUB338 and DAPI total cell-staining technology were used to research a fluorescence in situ hybridization experimental method for active sludge.By changing pre-treatment procedure and type of fixative,a series of ideal pictures of fluorescence in situ hybridization were obtained,in which the size,shape and distribution of the hybridized cells can be discerned clearly.However,the cells hybridized by probe EUB338 were not entirely in accordance with the cells stained by DAPI,so the e... |
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| Keywords: | Active sludge fixation hybridization auto fluorescence |
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