蚕豆根尖微核技术的方法学新论

虽然蚕豆根尖微核技术已是一个成熟规范的检测污染物遗传毒性的方法,但其中仍涉及一些重要的方法学问题值得深入探讨和研究。本文在图示正确观测蚕豆根尖细胞内微核和染色体畸变,如染色体断裂、丢失及染色体桥等的基础上,提出应该对蚕豆根尖分裂相细胞及其染色体畸变进行观测,以便更加准确、细致地反映污染物作用的剂量-效应关系和分子机制。同时还就该方法的其他重要问题提出了自己的观点,供同行们深入探讨和研究,使之不断完善,更好地服务于环境监测和风险评价等领域。

New Views on the Methodology of Micronucleus Assay with Vicia faba Root Tip Cells

Although the micronucleus assay with Vicia faba root tip cells is a canonical way to detect the genotoxicity of environmental pollutants, there are still some important issues that are worth in-depth discussion and investigation in its methodology. After illustrating how to correctly observe the micronucleus and chromosome aberrations, including chromosome fragment, chromosome loss and chromosome bridge, in the Vicia faba root tip cells we suggested that the dividing-phase cells and their chromosome aberrations should been taken account of in this assay, in order to more precisely and delicately determine the dose-effect relationship and potential molecular mechanisms than before. Other important issues about its methodology were also presented for our colleagues to discuss and explore deeply. The aim is to improve this assay to better serve the environmental monitoring and risk assessment.