| 固相萃取-高效液相色谱法测定武汉东湖水体中微囊藻毒素 |
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| 引用本文： | 贺小敏,李爱民,杨登,马先锋,宋国强. 固相萃取-高效液相色谱法测定武汉东湖水体中微囊藻毒素[J]. 中国环境监测, 2012, 28(1): 53-56 |
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| 作者姓名： | 贺小敏 李爱民 杨登 马先锋 宋国强 |
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| 作者单位： | 湖北省环境监测中心站, 湖北 武汉 430072;湖北省环境监测中心站, 湖北 武汉 430072;湖北省环境监测中心站, 湖北 武汉 430072;湖北省环境监测中心站, 湖北 武汉 430072;湖北省环境监测中心站, 湖北 武汉 430072 |
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| 摘 要： | 建立了固相萃取-高效液相色谱法(SPE-HPLC)测定东湖水样中微囊藻毒素(MC)的方法,考察了不同流动相、淋洗液和洗脱液对MC-RR和MC-LR测定的影响。结果表明,MC-RR和MC-LR的方法检出限分别为0.0789μg/L和0.0234μg/L,其线性定量范围分别为0.1～10.0mg/L和0.06～10.0mg/L;样品测定回收率为78.4%～97.4%,RSD小于6.3%。该法灵敏度高,快速准确,用于实际水样测定的结果令人满意。
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| 关 键 词： | 高效液相色谱 固相萃取 微囊藻毒素 东湖 |
| 修稿时间： | 2010-11-02 |
Determination of Microcystins in Donghu Lake by SPE-HPLC |
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| HE Xiao-min. Determination of Microcystins in Donghu Lake by SPE-HPLC[J]. Environmental Monitoring in China, 2012, 28(1): 53-56 |
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| Authors: | HE Xiao-min |
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| Affiliation: | Hubei Province Environmental Monitoring Centre, Wuhan 430072, China;Hubei Province Environmental Monitoring Centre, Wuhan 430072, China;Hubei Province Environmental Monitoring Centre, Wuhan 430072, China;Hubei Province Environmental Monitoring Centre, Wuhan 430072, China;Hubei Province Environmental Monitoring Centre, Wuhan 430072, China |
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| Abstract: | Microcystins(MC-RR and MC-LR) in Donghu Lake were determined by high performance liquid chromatograph(HPLC) with solid phase extraction(SPE).The optimum condition of determination by HPLC and the elution solutions for sample preparation were discussed.Determination limits of the method were 0.0789μg/L and 0.0234μg/L,and linear arranges were 0.1~10.0mg/L and 0.06~10.0mg/L for MC-RR and MC-LR,respectively.Recoveries of microcystins were from 78.4%to 97.4%.The RSD of determination was lower than 6.3%.The method offers traits of high sensitivity,speediness and exactness,and was applied to the determination of MC-RR and MC-LR in water samples with satisfactory results. |
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| Keywords: | HPLC SPE Microcystins Donghu Lake |
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