Characterization of a sex pheromone in a simultaneous hermaphroditic shrimp, Lysmata wurdemanni |
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Authors: | Dong Zhang Junda Lin Maggy Harley Jörg D Hardege |
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Institution: | (1) School of Environmental Science and Public Health, Wenzhou Medical College, 325035 Wenzhou, People’s Republic of China;(2) Vero Beach Marine Laboratory, Florida Institute of Technology, 805 46th Place East, Vero Beach, FL 32963, USA;(3) Department of Biological Sciences, Hull University, Cottingham Road, Hull, HU6 7RX, UK |
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Abstract: | Olfactory chemical cues have been described to play important roles in the control of mate recognition in many decapod crustaceans.
However, we still know very little about the chemical characteristics of the cues that coordinate pre-copulative behaviour.
In this study, we partially characterized a waterborne sex pheromone of a marine shrimp, Lysmata wurdemanni. Female moulting water was collected and ultrafiltered using 1,000 and 500-Da membranes, respectively, and analysed using
HPLC with a Lichrosphere™ RP18 (C18) column. The sex pheromone is likely to be a molecule between 500 and 1,000 Da in size
because behavioural bioassays showed that males responded to the supernatant of 500-Da and to the 1,000-Da filtrate, but did
not respond to the supernatant of 1,000-Da or to the 500-Da filtrate. There was only one dominant peak (2.86 min) detectable
in HPLC chromatograms of the supernatant of the 500-Da filtration. This peak showed a UV absorbance maximum at 274 nm, similar
to the recently identified shore crab sex pheromone Uridine-di-phosphate (UDP). Behavioural bioassays confirmed that this
peak is a bioactive component of a potential pheromone bouquet, but is different from UDP, which showed no bioactivity in
Lysmata wurdemanni. Our results lay the foundation for future studies to purify and eventually identify this sex pheromone. |
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