Ectopic expression of Arabidopsis ABC transporter MRP7 modifies cadmium root-to-shoot transport and accumulation |
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Authors: | Sylwia Wojas Sonia Plaza Oskar Siemianowski Anna Ruszczyńska Danuta M Antosiewicz |
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Institution: | a Faculty of Biology, University of Warsaw, Miecznikowa str. 1, 02-096 Warszawa, Poland b Institute of Plant Biology, University of Zürich, CH-8008 Zürich, Switzerland c Institute of Biochemistry and Biophysics PAS, Pawinskiego str. 5A, 02-106 Warszawa, Poland d Faculty of Chemistry, University of Warsaw, Pasteura str.1, 02-093 Warszawa, Poland |
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Abstract: | Arabidopsis MRPs/ABCCs have been shown to remove various organic and inorganic substrates from the cytosol to other subcellular compartments. Here we first demonstrate that heterologous expression of AtMRP7 in tobacco (Nicotiana tabacum var. Xanthi) modifies cadmium accumulation, distribution and tolerance. Arabidopsis MRP7 was localized both in the tonoplast and in the plasma membrane when expressed in tobacco. Its overexpression increased tobacco Cd-tolerance and resulted in enhanced cadmium concentration in leaf vacuoles, indicating more efficient detoxification by means of vacuolar storage. Heterologous AtMRP7 expression also led to more efficient retention of Cd in roots, suggesting a contribution to the control of cadmium root-to-shoot translocation. The results underscore the use of AtMRP7 in plant genetic engineering to modify the heavy-metal accumulation pattern for a broad range of applications. |
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Keywords: | ABC transporter Cadmium AtMRP7 Phytoremediation Plants tobacco |
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