| 外源水杨酸对梨叶片感染轮纹病菌后抗氧化酶活性及Ca2+浓度的影响 |
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| 引用本文： | 刘招龙,张绍铃,高富永.外源水杨酸对梨叶片感染轮纹病菌后抗氧化酶活性及Ca2+浓度的影响[J].应用与环境生物学报,2011(2):215-218. |
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| 作者姓名： | 刘招龙 张绍铃 高富永 |
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| 作者单位： | 南京农业大学园艺学院;宁德师范学院; |
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| 基金项目： | 国家自然科学基金项目(No.30170651)资助~~ |
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| 摘 要： | 为了解水杨酸(Salicylic acid,SA)缓解轮纹病菌伤害植物的机理,用0.3 mmol L-1的SA预先处理梨叶片,3 d后接种轮纹病菌(Physalospora Piricola Nose),接种4 d后开始测定各种指标,随后每隔3 d测定1次,共5次.结果表明,经0.3 mmol L-1 SA处理后,梨叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、愈创木酚过氧化物酶(GPX)和谷胱甘肽还原酶(GR)的活性分别比对照组(CK1)提高了30.65%、25.36%、28.55%、21.11%、35.67%和26.45%.电镜观察表明,感染轮纹病菌的梨叶片,Ca2+较多分布在细胞膜内侧、细胞质及一些细胞器(叶绿体)上.经SA处理后感染轮纹病菌的梨病叶,Ca2+较多分布在细胞壁外侧及细胞间隙中,与正常梨叶片Ca2+的分布情况相似.表明较低浓度的SA可缓解轮纹病菌对梨叶片的伤害作用.
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| 关 键 词： | 砂梨 轮纹病菌 水杨酸 抗氧化酶 Ca2+. |
Effect of Exogenous Salicylic Acid on Antioxidant Enzymes and Ca~(2+) Density in Pear Leaves Infected by Physalosproa piricola Nose |
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| LIU Zhaolong,ZHANG Shaoling & GAO Fuyong.Effect of Exogenous Salicylic Acid on Antioxidant Enzymes and Ca~(2+) Density in Pear Leaves Infected by Physalosproa piricola Nose[J].Chinese Journal of Applied and Environmental Biology,2011(2):215-218. |
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| Authors: | LIU Zhaolong ZHANG Shaoling & GAO Fuyong |
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| Institution: | LIU Zhaolong1,2,ZHANG Shaoling1 & GAO Fuyong1(1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)(2Ningde Normal University,Ningde 352100,Fujian,China) |
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| Abstract: | alicylic acid,a plant induced-resistance activator,is able to alleviate the injury of pathogen to plant.In this study,0.3 mmol L-1 salicylic acid(SA) was applied on the leaves of pear,after 3 d Physalosproa piricola Nose was inoculated,and 4 d after inoculation all indexes were measured.The measurement was made every 3 days for 5 times.The results showed that compared with CK1,the 0.3 mmol L-1 SA could increase the activities of SOD,POD,CAT,APX,GPX and GR proline in leaves by 30.65%,25.36%,28.55%,21.11%,35.... |
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| Keywords: | Pyrus serotina Rehd Physalospore piricola Nose salicylic acid antioxidant enzyme Ca2 |
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