| 耐盐异养硝化菌驯化方法及分离菌株鉴定 |
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| 引用本文： | 张培玉,曲洋,杨瑞霞,郭沙沙,于德爽.耐盐异养硝化菌驯化方法及分离菌株鉴定[J].应用与环境生物学报,2011(1):121-125. |
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| 作者姓名： | 张培玉 曲洋 杨瑞霞 郭沙沙 于德爽 |
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| 作者单位： | 青岛大学环境科学与工程系; |
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| 基金项目： | 国家自然科学基金项目(Nos.50678085,50878107); 青岛大学优秀研究生学位论文培育项目(No.YSPY2009014)资助~~ |
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| 摘 要： | 采用高C/N、逐渐降低DO、菌体对数生长期前期转接以及增加培养液中海水比例的方法完成了耐盐异养硝化菌的富集和驯化,并从中分离筛选出两株高效异养硝化菌qy37和gs2.通过对两株菌的形态观察、生理生化试验以及16S rRNA序列分析,确定菌株qy37和菌株gs2分别为假单胞菌属(Pseudomonas)和盐单胞菌属(Halomonas).
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| 关 键 词： | 富集 驯化 异养硝化菌 同步脱氮 耐盐 污水处理 |
Domestication of Halotolerant Heterotrophic Nitrifying Bacteria and Identification of Two Isolated Strains |
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| ZHANG Peiyu,QU Yang,YANG Ruixia,GUO Shasha & YU Deshuang.Domestication of Halotolerant Heterotrophic Nitrifying Bacteria and Identification of Two Isolated Strains[J].Chinese Journal of Applied and Environmental Biology,2011(1):121-125. |
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| Authors: | ZHANG Peiyu QU Yang YANG Ruixia GUO Shasha & YU Deshuang |
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| Institution: | ZHANG Peiyu,QU Yang,YANG Ruixia,GUO Shasha & YU Deshuang(Department of Environmental Science and Engineering,Qingdao University,Qingdao 266071,China) |
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| Abstract: | A new method for enrichment and domestication of halotolerant heterotrophic nitrifying bacteria was studied.The enrichment and domestication were accomplished by adopting high C/N ratio,gradually reducing DO,inoculating domestic bacteria to new culture medium in log phase and increasing the proportion of sea water for culture medium.Furthermore,two trains of heterotrophic nitrifying bacteria,qy37 and gs2 were isolated from enrichment and domestication system.Based on their morphological and physiological ch... |
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| Keywords: | enrichment domestication heterotrophic nitrifying bacterium simultaneous nitrogen removal halotolerant sewage disposal |
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