| 反应条件对苛求芽孢杆菌胞内尿酸酶与嘌呤衍生物作用的影响 |
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| 引用本文： | 李想,冯娟,张纯,蒲军,杨晓兰,袁拥华,廖飞.反应条件对苛求芽孢杆菌胞内尿酸酶与嘌呤衍生物作用的影响[J].应用与环境生物学报,2011(1):91-94. |
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| 作者姓名： | 李想 冯娟 张纯 蒲军 杨晓兰 袁拥华 廖飞 |
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| 作者单位： | 重庆医科大学重庆生物化学与分子药理学重点实验室; |
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| 基金项目： | 国家自然科学基金项目(Nos.30672009,81071427); 教育部新世纪优秀人才支持计划(No.NCET-09)~~ |
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| 摘 要： | 用底物尿酸的紫外吸收变化跟踪反应,用单或双底物米氏方程和初速度估算苛求芽孢杆菌胞内尿酸酶最大反应速度,按Arrenius经验公式得到该尿酸酶在pH 9.2和pH 7.4的活化能,但这些活化不能解释其在两个pH下的催化活性差异.该酶同常见嘌呤衍生物氧嗪酸、黄嘌呤和尿酸有亲和力,但对尿酸亲和力最低,且亲和力与嘌呤衍生物解离成阴离子的能力相关.在生理条件下,该酶对尿酸的亲和力相对于最适条件有显著下降.据此推测,优化静电相互作用选择性增强该酶对尿酸的亲和力是提高其成药性的可能措施之一.
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| 关 键 词： | 尿酸酶 嘌呤衍生物 尿酸 苛求芽孢杆菌 活化能 亲和力 |
Effects of Reaction Conditions on Interactions of Some Purine Derivatives with the Intracellular Uricase from Bacillus fastidiosus |
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| LI Xiang,FENG Juan,ZHANG Chun,PU Jun,YANG Xiaolan,YUAN Yonghua & LIAO Fei.Effects of Reaction Conditions on Interactions of Some Purine Derivatives with the Intracellular Uricase from Bacillus fastidiosus[J].Chinese Journal of Applied and Environmental Biology,2011(1):91-94. |
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| Authors: | LI Xiang FENG Juan ZHANG Chun PU Jun YANG Xiaolan YUAN Yonghua & LIAO Fei |
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| Institution: | LI Xiang,FENG Juan,ZHANG Chun,PU Jun,YANG Xiaolan,YUAN Yonghua & LIAO Fei(Chongqing Key Laboratory of Biochemical and Molecular Pharmacology,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | By monitoring the changes of UV absorbance of uric acid,the activation free energies of the intracellular uricase from Bacillus fastidiosus at pH 9.2 and pH 7.4 were estimated with Arrhenius equation,and the maximal reaction rates approximated from initial rates,but they can not account for the difference in the catalytic activity of this uricase under the two pH values.Among common purine derivatives,oxonate,xanthine and uric acid had affinities to this uricase,and uric acid had the lowest affinity.These a... |
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| Keywords: | uricase purine derivatives uric acid Bacillus fastidiosus activation free energy affinity |
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