| 青稞B-hordein基因表达载体构建及对小麦的遗传转化 |
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| 引用本文： | 赵高梅,龙海,邓光兵,潘志芬,余懋群.青稞B-hordein基因表达载体构建及对小麦的遗传转化[J].应用与环境生物学报,2011(3):364-368. |
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| 作者姓名： | 赵高梅 龙海 邓光兵 潘志芬 余懋群 |
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| 作者单位： | 中国科学院成都生物研究所;中国科学院研究生院; |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项(No.2008ZX08009-003); 国家科技基础性工作专项(No.2006FY110700)资助~~ |
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| 摘 要： | 利用从具有特殊B-hordein亚基组成的青藏高原青稞材料中克隆的B-hordein编码基因(SL60)和小麦高分子量谷蛋白亚基胚乳特异表达启动子PGlu1Dx构建了真核表达载体pCB2007-SL60.通过农杆菌介导对普通小麦进行遗传转化,共获得227株再生植株,经PCR鉴定和转化片段测序验证,最终获得5株阳性植株.研究为进一步分析B-hordein基因在小麦背景中的遗传和表达,以及对小麦加工特性的影响奠定了基础.图5表2参21
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| 关 键 词： | 青稞 B组醇溶蛋白 载体构建 转基因 小麦 GATEWAY |
Vector Construction and Genetic Transformation of B-hordein Gene from Hulless Barley to Wheat |
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| ZHAO Gaomei,LONG Hai,DENG Guangbing,PAN Zhifen & YU Maoqun.Vector Construction and Genetic Transformation of B-hordein Gene from Hulless Barley to Wheat[J].Chinese Journal of Applied and Environmental Biology,2011(3):364-368. |
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| Authors: | ZHAO Gaomei LONG Hai DENG Guangbing PAN Zhifen & YU Maoqun |
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| Institution: | ZHAO Gaomei1,2,LONG Hai1,DENG Guangbing1,PAN Zhifen1 & YU Maoqun1(1Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China)(2Graduate University of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | By the GATEWAY? strategy,a eukaryotic expression vector(pCB2007-SL60) was constructed to express a B-hordein gene isolated from hulless barley in the Qinghai-Tibetan Plateau,China.It was driven by an endosperm-specific promoter of high-moleculer-weight glutenin subunit of wheat and was transformed by Agrobacterium-mediated method in wheat.Totally,227 transgenic plants were obtained,and of them 5 positive plants were screened by means of PCR amplification.It was a key step to study the expression and heredit... |
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| Keywords: | hulless barely B-hordein vector construction gene transformation wheat GATEWAY? |
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