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苯酚降解菌UW7的鉴定及对苯酚的降解作用
引用本文:刘国生,郝晓洁,段佩玲,侯瑛,秦文艳.苯酚降解菌UW7的鉴定及对苯酚的降解作用[J].应用与环境生物学报,2011(1):118-120.
作者姓名:刘国生  郝晓洁  段佩玲  侯瑛  秦文艳
作者单位:河南师范大学生命科学学院;河南省滑县环境保护局;
基金项目:国家科技部科技人员服务企业行动项目(No.2009GJD00044); 河南省教育厅科技攻关项目(No.200761004)资助~~
摘    要:从焦化厂污水处理曝气池泥样中分离出具有降解苯酚能力的UW7菌株,根据形态、生理生化性状初步鉴定为不动杆菌属(Acinetobacter).该菌16S rRNA基因序列(在GenBank中的登录号为GU083586)与多株鲁氏不动杆菌(A.lowffii)的相似性在99%以上.结合形态、生理生化特性,鉴定UW7菌株为鲁氏不动杆菌(A.lowffii UW7),该种细菌具有降解苯酚的特性尚未见报道.该菌株降解苯酚的最适温度为30℃,最适生长pH 7.0,对2.5 g/L浓度的苯酚能够有效降解,对3.5~4.0 g/L浓度的苯酚有较强的耐受能力,是处理高浓度苯酚废水的良好菌种资源.

关 键 词:鲁氏不动杆菌  苯酚降解菌  16S  rDNA序列分析  生物降解  废水处理

Identification and Characteristics of a Phenol-degrading Bacterium UW7
LIU Guosheng,HAO Xiaojie,DUAN Peiling,HOU Ying & QIN Wenyan.Identification and Characteristics of a Phenol-degrading Bacterium UW7[J].Chinese Journal of Applied and Environmental Biology,2011(1):118-120.
Authors:LIU Guosheng  HAO Xiaojie  DUAN Peiling  HOU Ying & QIN Wenyan
Institution:LIU Guosheng1,HAO Xiaojie1,DUAN Peiling1,HOU Ying1 & QIN Wenyan2(1College of Life Sciences,Henan Normal University,Xinxiang 453007,Henan,China)(2Huaxian Agency of Environmental Protection,Anyang 456400,China)
Abstract:A bacterial strain capable of degrading phenol,designated UW7,was isolated from activated sludge of a wastewater treatment plant in a coking chemical factory.Strain UW7 was identified as Acinetobacter lowffii according to its morphological,physiological and biochemical properties,and the analysis of its 16S rRNA gene sequence that showed more than 99% similarity to several strains of A.lowffii.A strain of A.lwoffii able to degrade phenol had never been reported.The optimum pH and temperature for its growth ...
Keywords:Acinetobacter lwoffii  phenol-degrading bacterium  16S rDNA sequence alignment  biodegradation  wastewater treatment  
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