高效液相色谱法测定水和废水中的DMF、DMAC、DMSO研究

建立了高效液相色谱法测定水样中N,N-二甲基甲酰胺(DMF)、N,N-二甲基乙酰胺(DMAC)和二甲基亚砜(DMSO)含量方法.本方法采用Venusil MP C18色谱柱,流动相乙腈:水(6:94,v/v),测定波长为:DMF与DMAC 220 nm;DMSO 210 nm.目标物在约0.5~10 mg·L-1范围内呈良好线性,线性相关系数r在0.999以上,检出限分别为:DMF与DMAC 0.02 mg·L-1、DMSO 0.1 mg·L-1,加标回收率在97%~109%,相对标准偏差RSD≤2%.本法前处理简单、有机溶剂消耗少,操作方便快捷.

Determination of 2-Methylimidazole and 4-Methylimidazolein Imidazole by High Performance Liquid Chromatography

A high performance liquid chromatography method was developed for the determination of 2-Methylimidazole and 4-Methylimidazole in Imidazole.The chromatography separation was performed on a XDB C18 column(4.6 mmi.d.× 250 mm,5 μm) using methanol-0.05 mol/L potassium dihydrogen hposphate buffer containing 0.05 mol/L heptanesulfonicacidsodium(5/95, v/v;pH3.5) as the mobile phase and the detection was at wave-length of UV-210nm.Good linearities were obtained for 2-methylimidazole and 4-methylimidazole at the concentration range of 0.10~25.0 mg·L-1 with relative coefficients(r) not less than 0.999 9.The limits of detection were 20 mg·kg-1 and 40 mg·kg-1 for 2-methylimidazole and 4-methylimidazole.The recoveries of target compound at 3 different concentration levels were in the range of 85%~105% with RSDs lower than 3%.This method has the advantage of simple pretreatment, less organic solvent consumption and convenient operation.