| 紫贻贝消化腺淀粉酶的提取及部分性质研究 |
| |
| 引用本文： | 王文霞,朱启忠,陈明台,冯连伟. 紫贻贝消化腺淀粉酶的提取及部分性质研究[J]. 资源开发与市场, 2010, 26(8): 678-680 |
| |
| 作者姓名： | 王文霞 朱启忠 陈明台 冯连伟 |
| |
| 作者单位： | 山东大学威海分校,海洋学院,山东,威海,264209 |
| |
| 基金项目： | 山东大学威海分校科研立项项目 |
| |
| 摘 要： | 以紫贻贝为原料,从其消化腺中提取淀粉酶,确定其最佳提取条件,并探讨其部分酶学性质。采用缓冲液提取法,通过单因素试验考察提取温度、提取溶剂的pH和提取时间三个因素对紫贻贝消化腺中淀粉酶活性的影响。用平行对照试验来研究该酶的最适催化温度以及各种离子对其活性的影响。结果表明,该酶的最佳提取条件为温度20℃、pH值3.0-4.0、提取时间45min;最适反应温度为35℃,EDTA、Mg2+、Zn2+对该酶有抑制作用,而Ca2+、Mn2+对该酶有促进作用。
|
| 关 键 词： | 紫贻贝 淀粉酶 提取条件 性质 |
Study on Extraction and Characters of Amylase in Mytilus edulis Digestive Gland |
| |
| WANG Wen-xia,ZHU Qi-zhong,CHEN Ming-tai,FENG Lian-wei. Study on Extraction and Characters of Amylase in Mytilus edulis Digestive Gland[J]. Resource Development & Market, 2010, 26(8): 678-680 |
| |
| Authors: | WANG Wen-xia ZHU Qi-zhong CHEN Ming-tai FENG Lian-wei |
| |
| Affiliation: | WANG Wen-xia,ZHU Qi-zhong,CHEN Ming-tai,FENG Lian-wei(Marine College,Shandong University at Weihai,Weihai 264209,China) |
| |
| Abstract: | In this paper,the conditions for extracting amylase from the digestive gland of Mytilus edulis and some characters of the amylase were studied.The optimal methodology for extracting was set up.Buffer would be used as extraction solvent.In the extraction procedure,the influence of three factors on the activity of amylase,which included extraction temperature,the pH of the extraction solvent and extraction time,would be sdudied through single factor experiment.By use of parallel controlled trial,the best cata... |
| |
| Keywords: | Mytilus edulis amylase extraction conditions character |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
