极低频电磁场对萌发期大豆的生物学效应

观察了50Hz.0.2mT或6.0mT的电磁场暴露对大豆的萌发过程，丙二醛（MDA）含量，超氧化物歧化酶（SOD），过氧化氢酶（CAT），过氧化物酶（POD），蛋白酶活性和可溶性蛋白含量以及幼苗根茎的组织形态学影响。结果表明：电磁场暴露下，大豆的开始萌发时间由32h提前到8h，萌发率到达50％的时间由72h分别提前到48h(0.2mT组）和40h(6.0mT组）；MDA含量随磁场强度的增大而下降；SOD，POD活性随磁场强度的增大而升高，而CAT活性在0.2mT暴露组下降，在6.0mT暴露组升高；蛋白酶活性和可溶性蛋白含量分别随磁场强度的增大升高或降低。幼苗根茎细胞的大小和形态没有明显变化。结果提示，极低频电磁场可以改变抗氧化酶的活性，减缓脂质过氧化，增强蛋白酶的活性，促进大豆的萌发过程。图4参6。

BIOLOGICAL EFFECTS OF EXTREMELY LOW FREQUENCY ELECTROMAGNETIC FIELDS ON SOYBEAN SEEDS DURING GERMINATION

Soybean seeds during germination were exposed to a sinusoidal 0.2 mT or 6.0 mT electromagnetic field(EMF) at 50 Hz. The effects of EMFs on germination ratio, malonaldehyde (MDA) content, the activities of superoxide dismutase (SOD), catalase (CAT),peroxide dismutase (POD) and proteinase, soluble protein content in soybean seeds, and the morphological structure of soybean seedlings were measured. The results indicated that the time germination started and germination percentage got to 50% was earlier for the seeds exposed to EMFs than for the control. MDA content decreased and the activities of SOD and POD increased with the increase of magnetic field intensity. The activities of CAT decreased in 0.2 mT field but increased in 6.0 mT field. Proteinase activity increased and soluble protein content decreased with the increase of magnetic field intensity. No significant difference induced by EMFs was observed in the morphological structure of soybean seedlings. These results suggested that 50 Hz EMFs could increase the activities of anti oxidation enzymes, alleviate lipid per oxidation, enhance proteinase activity and therefore accelerate the germination of soybean seeds. Fig 4, Ref 6