| 朱鹮的随机扩增多态DNA分析与种内亲缘关系研究 |
| |
| 引用本文： | 刘斌,韩之明,刘彦,李福来.朱鹮的随机扩增多态DNA分析与种内亲缘关系研究[J].应用与环境生物学报,1999,0(1):40. |
| |
| 作者姓名： | 刘斌 韩之明 刘彦 李福来 |
| |
| 作者单位： | 1. 北京动物园,北京,100044
2. 北京师范大学生物学系,北京,100875 |
| |
| 基金项目： | 北京市市政管理委员会资助项目，北京市园林局资助项目 |
| |
| 摘 要： | 利用随机扩增多态DNA技术对朱鹮 ( Nipponia nippon ) 8个个体进行了随机扩增多态DNA分析. 用20个10bp的随机引物对每只朱鹮的基因组DNA进行扩增,共得到168个扩增片段,其中共有片段为102个. 根据聚类分析所得到的树状图确定了8只朱鹮的亲缘关系,这为进一步构建全部朱鹮个体的谱系关系图打下了基础,有利于制定更有效的朱鹮保护计划.
|
| 关 键 词： | 朱鹮 基因组DNA 随机扩增多态DNA |
| 修稿时间： | 1998-08-18 |
RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS AND THE INTRASPECIFIC RELATIONSHIP OF CRESTED IBIS(NIPPONIA NIPPON) |
| |
| LIU Bin,HAN Zhiming,LIU Yan,LI Fulai.RANDOMLY AMPLIFIED POLYMORPHIC DNA ANALYSIS AND THE INTRASPECIFIC RELATIONSHIP OF CRESTED IBIS(NIPPONIA NIPPON)[J].Chinese Journal of Applied and Environmental Biology,1999,0(1):40. |
| |
| Authors: | LIU Bin HAN Zhiming LIU Yan LI Fulai |
| |
| Abstract: | Randomly amplified polymorphic
DNA (RAPD) was used to investigate genetic relationships of 8 crested ibises (Nipponia
nippon). Twenty arbitrary primers were used to amplify genomic DNA of each ibis. Among
them, 17 primers had clear and distinct RAPD patterns in each amplification.168 amplified
fragments were generated by RAPD and 102 fragments were shared by the 8 ibises.
Hierachical cluster analysis of the RAPD data was used to calculate the similarity
coefficients by spss/pc+ programme. Based on the correlation similarity coefficient
matrix, a dendrogram was constructed. The dendrogram indicates the relationships of the 8
crested ibises. This study provides the basis for constructing the relationship dendrogram
of all individuals, which is helpful in working out a better conservation plan. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
|
