茶叶挥发物对白斑猎蛛觅食行为的影响

为了探明茶树叶片挥发物在白斑猎蛛(Evarcha albaria)选择觅食斑块过程中的作用,采用固相微萃取-气相色谱-质谱联用技术研究了其猎物假眼小绿叶蝉(Empoasca vitis)取食对茶树叶片挥发物释放的影响,同时利用蜘蛛行为观测平台研究了白斑猎蛛在不同味源侧(觅食斑块)的活动行为。结果表明,未损伤叶片(对照)仅释放出微量挥发物,而假眼小绿叶蝉成虫取食后的茶树叶片释放出14种挥发物;雌、雄蛛在进入处理味源(假眼小绿叶蝉取食后的茶树叶片)侧和对照侧的初次选择及进入频次上均无显著差异,但与对照相比,雌、雄蛛在虫害处理叶片中的停留时间显著增加。研究表明,假眼小绿叶蝉取食诱导了茶树叶片挥发物的释放,而挥发物种类和含量的变化造成了白斑猎蛛在不同味源侧停留时间的差异。

Effects of Tea Volatiles on Foraging Behavior of Evarcha albaria

To understand effects of tea volatiles on foraging behavior of jumping spiders,Evarcha albaria,the technology of solid phase micro-extraction-gas chromatography-mass spectrometry(SPME-GC-MS) was used to first study effect of leaf-feeding of its preys,false-eye leafhoppers(Empoasca vitis),on release of volatiles from affected tea leaves and at the same time observation was made of foraging behavior of jumping spiders beside different odor sources(or in foraging patches) on a spider behavior observation platform,It was found that intact tea leaves released only a minute amount of volatiles,while Empoasca vitis-bitten tea leaves gave out 14 kinds of volatiles.Once placed near different odor sources,control(intact leaf) or treatment(bitten leaf) jumping spiders,regardless of male or female,did not show much preference to control or treatment in their first choice or much difference in frequency of their moving into foraging patches affected by different odor sources.However,they stayed significantly longer in the patch of E.vitis-bitten tea leaves.The findings suggested that E.vitis biting of tea leaves induced release of volatiles from the leaves and the change in kind and content of the volatiles,which led to the difference in duration of E.albaria staying in different foraging patches.