一株脱硫菌的鉴定及其对H2S的降解能力

取污水处理厂有生物活性的污泥,经富集培养、分离纯化得到1株脱硫活性较高的菌株CTD843-T-3,将其载入生物滴滤塔中对H2S进行连续脱硫反应.当最佳反应温度为30℃、pH为6.0、H2S进口浓度为500～3000mg·m-3时,菌株对H2S的去除率可保持在92％以上,H2S最高负荷率可达6.5g·m-3·h-1.通过形态生理特征和16SrDNA序列分析对CTD843-T-3进行鉴定,结果表明该菌株为椭球状,大小为0.4～0.6μm,为革兰阴性菌,属假单胞菌属(Pseudomonas sp.).

Identification of a Desulfurizing Strain and Its H2S Degrading Capacity

An active desulfurizing strain of bacteria CTD843-T-3 was isolated from bio-active sludge collected in a wastewater treatment plant and loaded into a bio-trickling reactor for consecutive desulfurization of H2S.When the reaction temperature was adjusted to 30 ℃,pH to 6.0,and the inlet concentration of H2S to 500-3 000 mg·m-3,the H2S removal rate of the strain could be kept over 92%,and the maximum H2S loading rate could reach 6.5 g·m-3·h-1.Through the morphological,biochemical & physiological identification,and 16S rRNA sequence analysis,the strain was found to be ellipse-shaped,0.4-0.6 μm in size,gram-negative,and a member of Pseudomonas.