Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide |
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Authors: | G R Seely M J Duncan W E Vidaver |
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Institution: | (1) Present address: C.F. Kettering Research Laboratory, Yellow Springs, Ohio, USA;(2) Department of Biological Sciences, Simon Fraser University, Burnaby, Canada |
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Abstract: | Dimethyl sulfoxide (DMSO) rapidly extracts much of the chlorophyll c and fucoxanthin from the intact thalli of brown algae. Subsequent extraction by acetone rapidly removes most of the chlorophyll a and -carotene. An analytical procedure for these pigments has been developed, based upon this extraction sequence, followed by partition of the acetone extract between hexane and aqueous acetone. Fucoxanthin and chlorophyll c can be recovered from the DMSO extract and the aqueous acetone phase by extraction into ethyl acetate and subsequent evaporation. The DMSO-acetone extraction sequence is also effective with green algae and a few red algae, although the full scope of the method has not been investigated. |
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