| 氟对鲤鱼脑抗氧化系统及细胞凋亡的影响 |
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| 引用本文： | 曹谨玲,陈剑杰,王俊东,罗永巨. 氟对鲤鱼脑抗氧化系统及细胞凋亡的影响[J]. 环境科学学报, 2013, 33(3): 861-866 |
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| 作者姓名： | 曹谨玲 陈剑杰 王俊东 罗永巨 |
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| 作者单位： | 1. 山西农业大学动物科技学院,山西省生态畜牧与环境兽医学重点实验室,太谷030801
2. 广西水产研究所,南宁,530021 |
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| 基金项目： | 科技部国际合作项目(No.2010DFB30530-1);国家自然科学基金项目(No.31172376);山西省自然科学基金项目(No.2009021038);山西农业大学科研启动基金(No.XB2009003);山西农业大学博士后基金(No.92462);中国博士后科学基金(No.2012M520601) |
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| 摘 要： | 以鲤鱼为受试材料,研究了氟化钠对其脑组织的抗氧化系统、组织结构和细胞凋亡的影响.结果发现,染毒30 d时,随氟化钠浓度的升高,SOD、GSH活性表现为先诱导后抑制的趋势；暴露60 d与90 d后,SOD、GSH活性主要表现为抑制.而MDA水平在整个暴露实验期间均呈增高趋势.暴露90 d后,脑组织切片的显微观察结果显示,氟化钠能导致鲤鱼脑组织血栓形成及各细胞层结构发生病理学改变,并具有剂效相关性.生物检测结果显示,随着氟化钠暴露剂量增大,鱼脑细胞凋亡率的增加与MDA水平的升高呈正相关关系(r=0.9968),而Bcl-2的表达减弱与SOD、GSH活性呈正相关关系(r=0.9198,0.9889).
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| 关 键 词： | 氟化钠 鲤鱼 脑 抗氧化系统 细胞凋亡 |
| 收稿时间： | 2012-03-26 |
| 修稿时间： | 2012-05-14 |
The effects of fluorine on antioxidant system and apoptosis of brain tissue in carp (cyprinus carpio L.) |
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| CAO Jinling,CHEN Jianjie,WANG Jundong and LUO Yongju. The effects of fluorine on antioxidant system and apoptosis of brain tissue in carp (cyprinus carpio L.)[J]. Acta Scientiae Circumstantiae, 2013, 33(3): 861-866 |
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| Authors: | CAO Jinling CHEN Jianjie WANG Jundong LUO Yongju |
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| Affiliation: | Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801;Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801;Shanxi Key Laboratory of Ecological Animal Science and Environmental Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801;Guangxi Institute of Fisheries, Nanning 530021 |
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| Abstract: | The physiological toxicity of sodium fluoride on antioxidant system, organizational structure and apoptosis of brain tissue in Cyprinus carpio Linnaeus were studied. Results showed that along with the increasing concentration of sodium fluoride, the superoxide dismutase (SOD) and glutathione (GSH) activities increased firstly and then were inhibited after 30 days exposure. The SOD and GSH activities decreased after 60 and 90 days exposure. The content of lipid peroxides (MDA) increased during the whole test period. After 90 days exposure, histopathological changes of brain tissue were observed. It was found that thrombosis and structural changes in the cell layers were resulted from the exposure of sodium fluoride. The biological investigation results showed that there was a positive correlation between the cell apoptosis rate and the MDA levels (r=0.9968), but with dosage increasing, Bcl-2 protein concentration decreased, which was positive correlated with SOD and GSH activities (r=0.9198 and 0.9889, respectively). |
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| Keywords: | sodium fluoride carp brain antioxidant system apoptosis |
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