| 一株酸性大红3R脱色菌的分离、鉴定及其脱色性能研究 |
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| 引用本文： | 廖欢,严滨,黄国和,傅海燕,柴天,郭亚丽.一株酸性大红3R脱色菌的分离、鉴定及其脱色性能研究[J].环境科学学报,2013,33(3):691-699. |
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| 作者姓名： | 廖欢 严滨 黄国和 傅海燕 柴天 郭亚丽 |
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| 作者单位： | 1. 华北电力大学(北京)资源与环境研究院区域能源环境系统优化教育部重点实验室,北京102206;厦门理工学院环境工程系,厦门361024
2. 厦门理工学院环境工程系,厦门,361024 |
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| 基金项目： | 国家自然科学基金重大项目(No.51190095);教育部创新团队发展计划(No.IRT1127);福建省科技计划项目(No.2012J01236) |
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| 摘 要： | 通过浓度梯度驯化,从厌氧污泥中分离到一株酸性大红3R脱色菌M3,根据其形态学特征及16S rRNA基因序列分析,鉴定该厌氧菌株为阴沟肠杆菌(Enterobacter cloacae).同时,研究了该菌株在厌氧静置条件下对酸性大红3R的最适脱色条件.结果表明,菌株脱色的最佳营养源为酵母粉,最适宜脱色条件为酵母粉浓度1％、pH =6.5、温度35 ℃、盐度小于4％,在此条件下,对浓度为100 mg·L-1酸性大红3R溶液厌氧静置培养72 h,脱色率达到98％以上.菌株耐盐性良好,在6％ ～8％的盐度下具有较好的脱色效果.分光光谱表明,菌株脱色机制主要为生物降解脱色.毒性试验表明,脱色后的酸性大红3R毒性明显降低.
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| 关 键 词： | 酸性大红3R 分离 鉴定 阴沟肠杆菌 生物脱色 毒性试验 |
| 收稿时间： | 2012/5/20 0:00:00 |
| 修稿时间： | 2012/7/23 0:00:00 |
Isolation, identification and decoloring capability of an Acid Red 3R decoloring bacterial strain |
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| LIAO Huan,YAN Bin,HUANG Guohe,FU Haiyan,CHAI Tian and GUO Yali.Isolation, identification and decoloring capability of an Acid Red 3R decoloring bacterial strain[J].Acta Scientiae Circumstantiae,2013,33(3):691-699. |
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| Authors: | LIAO Huan YAN Bin HUANG Guohe FU Haiyan CHAI Tian and GUO Yali |
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| Institution: | 1. MOE Key Laboratory of Regional Energy and Environmental System Optimization, Resources and Environmental Research Academy, North China Electric Power University, Beijing 102206;2. Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024;Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024;1. MOE Key Laboratory of Regional Energy and Environmental System Optimization, Resources and Environmental Research Academy, North China Electric Power University, Beijing 102206;2. Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024;Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024;Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024;1. MOE Key Laboratory of Regional Energy and Environmental System Optimization, Resources and Environmental Research Academy, North China Electric Power University, Beijing 102206;2. Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024 |
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| Abstract: | |
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| Keywords: | Acid Red 3R isolation identification Enterobacter cloacae biological decoloring toxicity test |
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