| 荧光定量PCR技术在环境监测中的应用研究 |
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| 引用本文： | 赵晓祥,庞晓倩,庄惠生.荧光定量PCR技术在环境监测中的应用研究[J].环境科学与技术,2009,32(12). |
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| 作者姓名： | 赵晓祥 庞晓倩 庄惠生 |
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| 作者单位： | 东华大学环境科学与工程学院,上海,201620 |
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| 基金项目： | 国家自然基金项目资助，上海市重点学科建设项目资助 |
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| 摘 要： | 实时荧光定量PCR是在PCR基础上发展起来的一种核酸定量技术,此技术灵敏度高,特异性强。目前也已被国内外应用在环境监测方面。如可以用此方法来监测环境中微生物在河流中随季节的变化情况,评价菌的富集情况,快速检测微生物等。随着此技术的不断发展,此方法在环境监测方面将会有广阔的应用前景。
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| 关 键 词： | 荧光定量PCR 荧光探针 标准曲线 |
Application of Real-time Fluorescent Quantitative PCR in Environmental Monitoring |
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| ZHAO Xiao-xiang,PANG Xiao-qian,ZHUANG Hui-sheng.Application of Real-time Fluorescent Quantitative PCR in Environmental Monitoring[J].Environmental Science and Technology,2009,32(12). |
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| Authors: | ZHAO Xiao-xiang PANG Xiao-qian ZHUANG Hui-sheng |
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| Institution: | ZHAO Xiao-xiang,PANG Xiao-qian,ZHUANG Hui-sheng (School of Environmental Science and Engineering,Donghua University,Shanghai 201620,China) |
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| Abstract: | Real-time fluorescent quantitative PCR (FQ-PCR)which develops in the base of PCR technique as one kind of quantitative techniques about nucleic acid with high sensitivity and strong specificity was used in environmental monitoring to monitor the changes of microorganisms in rivers along with the seasons,estimate the enrichment of fungus and measure microorganisms quickly. With the development of PCR technology,the method will be widely applied in environmental monitoring in the future. |
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| Keywords: | real-time fluorescent quantitative PCR fluorescent probe standard curve |
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