Fabrication of Salinomycin and Biotin Encapsulated Polymeric Nanoparticles Showing Potent Antiproliferative Activity and Induce Apoptosis in Gastric Carcinoma Cells

Cytotoxicity can be much greater than all other treatments by targeted combination chemotherapy. In this context, we have recognized the use for gastric cancer chemotherapy of multifunctional polymer nanoparticles (PNPs) loaded with Salinomycin (SAL) and Biotin (BT). The encapsulation of BT and SAL anticancer agents co-loaded with polyethylene glycol (PEGs) and nanoparticles of polylactide-co-glycolides (PLGAs) is incompetent due to inadequacy in polymeric biodegradable nanoparticles (PNPs) of the dual antitumor drugs BT and SAL. The morphology of the BT@PNPs, SAL@PNPs and SAL/BT@PNPs, and composition and sizes of the nanoparticles was investigated using a transmission electron microscopy (TEM). In vitro cell death of human gastric cell lines including SGC-791 and NCI-N87 has also been caused by SAL/BT@PNPs. A variety of biochemical stains, for example, AO/EB (Acridine Orange / Ethidium Bromide) and Hoechst, have been used to detect morphological and cell death. The mechanical examination of apoptosis was further tested using the Annexin V-FITC flow cytometry. In overall, SAL/BT@PNPs may be used as a possible instrument for enhanced gastric cancer therapy efficacy in this dual approach to drug delivery.