| 拟南芥SHI基因在烟草中的表达和矮化、延迟开花的烟草植株的获得 |
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| 引用本文： | 彭世清,陈守才.拟南芥SHI基因在烟草中的表达和矮化、延迟开花的烟草植株的获得[J].应用与环境生物学报,2005,11(4):416-418. |
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| 作者姓名： | 彭世清 陈守才 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,海口,571101
2. 中国热带农业科学院热带生物技术研究所热带作物生物技术国家重点实验室,海口,571101;中国热带农业科学院橡胶研究所热带作物栽培生理农业部重点开放实验室,海南儋州,571737 |
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| 摘 要： | 利用聚合酶链式反应(PCR)从拟南芥基因组DNA中克隆了拟南芥SHOTINTERNODS(SHI)基因,并进行了序列测定.以载体pBI121为基本骨架构建了SHI的植物表达载体,并导入根癌农杆菌EHA105.通过农杆菌介导的叶盘法将拟南芥SHI基因转入烟草.PCR和RT-PCR检测证明,拟南芥SHI基因已整合到烟草基因组中并得以表达,获得了矮化、延迟开花的烟草植株.图5参12
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| 关 键 词： | 拟南芥 SHI基因 转化 矮化 烟草 |
| 收稿时间： | 2004-06-01 |
| 修稿时间： | 2004-07-23 |
EXPRESSION OF AN ARABIDOPSIS THALIANA SHI GENE IN TOBACCO AND OBTAIN OF DWARF AND LATE-FLOWERING PLANTS |
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| PENG Shiqing,CHEN Shoucai.EXPRESSION OF AN ARABIDOPSIS THALIANA SHI GENE IN TOBACCO AND OBTAIN OF DWARF AND LATE-FLOWERING PLANTS[J].Chinese Journal of Applied and Environmental Biology,2005,11(4):416-418. |
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| Authors: | PENG Shiqing CHEN Shoucai |
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| Abstract: | The SHI gene of Arabidopsis thaliana was cloned and sequenced. The plant expression vector with SHI was constructed and transformed into tobacco by Agrobacterium tumefaciens. Molecular identification was shown that the SHI gene was integrated into the genome of tobacco and expressed in transgenic tobaccos assayed by PCR and RT-PCR. The expression of an A. thaliana SHI gene in tobacco resulted in dwarf and late-flowering plants. Fig 5, Ref 12 |
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| Keywords: | Arabidopsis thaliana SHI gene transformation dwarfism tobacco |
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