Oxygen transport by the hemocyanin of the protobranch mollusc Solemya reidi |
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Authors: | N Sanders J J Childress B R McMahon |
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Institution: | (1) Truman State University, Kirksville, Missouri 63501, USA and Bamfield Marine Station, Bamfield, British Columbia VOR 1BO, Canada, CA;(2) Department of Biological Sciences, University of California, Santa Barbara, California 93106, USA, US;(3) Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N IN4, Canada, CA |
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Abstract: | Oxygen transport by the hemocyanin of the protobranch mollusc Solemya reidi Bernard was studied in native hemolymph samples. Clams were collected from two different reducing environments, beneath log
booms in Alberni Inlet, British Columbia, Canada, and from the sewage effulent in Santa Monica Bay, California, USA. The hemocyanin
concentration in a pooled hemolymph sample (n = 10 individuals) was 33.5 mg ml−1. The mean hemolymph pH of five Alberni clams maintained for 3 wk in mud was 7.96 ± 0.06. No significant variation in hemocyanin
oxygen-affinity or cooperativity was found for hemocyanin in whole hemolymph samples from these five individually studied
clams. There was a significant difference only at 15 °C in the oxygen affinity of hemocyanin in pooled whole-hemolymph samples
from S. reidi collected from Alberni Inlet compared with clams collected from Santa Monica Bay. Little effect of temperature on hemocyanin
oxygen-affinity was found for temperatures below 20 °C; above 20 °C, the oxygen affinity was reduced for clams from both sites.
Temperature and pH had no apparent affect on hemocyanin cooperativity. Moderate Bohr shifts were found at all temperatures
examined. The presence of physiologically relevant concentrations of thiosulphate in hemocyanin samples resulted in a decrease
in hemocyanin oxygen-affinity, opposite to the effect on hemocyanin found for the hydrothermal vent crab Byth-ograea thermydron, but thiosulphate had no effect on hemocyanin cooperativity.
Received: 2 September 1997 / Accepted: 6 February 1998 |
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