In vitro evaluation of cellular influences induced by stable fullerene C70 medium dispersion: Induction of cellular oxidative stress |
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| Institution: | 1. Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Japan 1-1 Iseigaoka, Kitakyushu, Fukuoka 807-8555, Japan;2. Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan;3. National Metrology Institute of Japan (NMIJ), AIST, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan;4. Research Institute of Science for Safety and Sustainability (RISS), AIST, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan;5. Research Institute for Environmental Management Technology (EMTECH), AIST, Particle Measurement Group, 16-1 Onogawa, Tsukuba, Ibaraki 305-8569, Japan;6. Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;1. I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS, 44 Thorez pr., St. Petersburg 194223, Russian Federation;2. Institute of Experimental Medicine RAMS, 12 Akademika Pavlova str., St. Petersburg 197376, Russian Federation;3. St. Petersburg State University, Universitetskayanab., 7-9, St. Petersburg 199034, Russian Federation;1. Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P. M. B. 12003 Lagos, Nigeria;2. Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P. M. B. 12003 Lagos, Nigeria;3. Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, P. M. B. 12003 Lagos, Nigeria;1. Department of Neurology, Stroke Center, Buffalo Medical Center, Buffalo, NY, USA;2. Department of Bioinformatics, Roswell Park Cancer Institute, Carlton Street, Buffalo, NY, USA;1. Department of Chemistry, University of Turku, FIN-20014 Turku, Finland;2. Department of Biochemistry/Biotechnology, University of Turku, FIN-20520 Turku, Finland;3. Radiometer Turku, Biolinja 12, FIN-20750 Turku, Finland |
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| Abstract: | Fullerene is one of the nanocarbons that is expected to have applications to life science, such as nanomedicines. An understanding of the cellular influences of fullerene is essential for its application to life science. Although C60 and C70 are both known as major fullerenes, most previous reports about the cellular influences of fullerene are about C60. Thus we evaluated the cellular influences caused by C70. A stable and uniform C70-medium dispersion was prepared. The dispersion was stable for the experimental period. Mitochondrial activity (MTT assay), colony forming ability (clonogenic assay), induction of oxidative stress (intracellular ROS and lipid peroxidation levels) and cellular uptake (TEM observation) in human keratinocyte HaCaT and lung carcinoma A549 cells exposed to C70 were examined. C70 did not influence mitochondrial activity. On the other hand, C70 dispersion inhibited colony formation at the concentration of 25.2 μg mL?1. Exposure to C70 dispersion caused an increase in intracellular ROS and lipid peroxidation levels. The induction of intracellular ROS level was inhibited by pre-treatment of the cells by antioxidants. TEM observations of C70 exposed cells showed cellular uptake of C70. These results were similar to the cellular influences caused by C60 which were reported by us previously. Although C70 did not cause cell death, it caused the induction of intracellular oxidative stress. |
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| Keywords: | Fullerene Oxidative stress Cytotoxicity Cellular uptake |
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