| 基于高通量测序技术的铬污染农田土壤菌群多样性及修复菌株的筛选 |
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| 引用本文： | 肖蓉,王媛,聂园军,程滨,赵佳,曹秋芬.基于高通量测序技术的铬污染农田土壤菌群多样性及修复菌株的筛选[J].应用与环境生物学报,2019(4):933-942. |
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| 作者姓名： | 肖蓉 王媛 聂园军 程滨 赵佳 曹秋芬 |
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| 作者单位： | 山西省农业科学院果树研究所;山西省农业科学院农业资源与经济研究所;山西省农业科学院农业环境与资源研究所;山西省农业科学院生物技术研究中心 |
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| 基金项目： | 山西省科技重点研发项目(201603D21110-1-6);山西省农业科技成果转化和示范推广项目(2017CGZH31)资助~~ |
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| 摘 要： | 菌种资源筛选是铬污染土壤生物修复的基础.以铬污染农田土壤为研究对象,采用高通量测序方法分析土壤细菌多样性特征,并根据多样性分析结果,采用选择性培养基和培养方法快速筛选对铬具有适应性和去除能力的细菌,以寻找能在铬污染农田土壤原位修复中具有较大应用潜力的菌种资源.结果显示:受铬污染的土壤细菌群落丰富度和多样性均低于未受铬污染土壤.门水平上,铬污染土壤中放线菌门(Actinobacteria)细菌丰度显著高于未受铬污染的土壤,是两种生境丰度差异最大的门.属水平上,芽孢杆菌属(Bacillus)是铬污染土壤中丰度最高的优势属,显著高于未受铬污染的土壤.从铬污染土壤中共分离到6株能够耐受1 000 μg/mL铬的细菌. 6株菌都具有一定的六价铬Cr(VI)去除能力,其中Cr1、Cr3和Cr8能在72 h内将500 μg/mL铬培养基中的Cr(VI)全部去除,Cr8能在72 h内将1 000 μg/mL铬培养基中的Cr(VI)去除61.2%. 16S rDNA测序结果表明,6株菌中有5株属于厚壁菌门芽孢杆菌属,1株属于放线菌门纤维微菌属(Cellulosimicrobium).结合菌体及菌落形态特征和序列分析结果,铬去除能力最高的Cr8菌可初步被确定为C. aquatile.本研究表明铬污染降低土壤中细菌多样性,根据高通量测序结果有选择性地快速筛选铬污染修复菌株具有可行性,Cr8菌是国内首次分离到的具有铬污染修复功能的纤维微菌属菌株.
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| 关 键 词： | 高通量测序 细菌多样性 耐铬 纤维微菌属 快速筛选 |
Research on bacterial communities in Cr-polluted farmland soil by highthroughput sequencing and selection of Cr remediation strains |
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| XIAO Rong,WANG Yuan,NIE Yuanjun,CHENG Bin,ZHAO Jia,CAO Qiufen.Research on bacterial communities in Cr-polluted farmland soil by highthroughput sequencing and selection of Cr remediation strains[J].Chinese Journal of Applied and Environmental Biology,2019(4):933-942. |
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| Authors: | XIAO Rong WANG Yuan NIE Yuanjun CHENG Bin ZHAO Jia CAO Qiufen |
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| Institution: | (Pomology Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;Institute of Agricultural Resources and Economy, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China;Institute of Agricultural Environment and Resource, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China) |
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| Abstract: | XIAO Rong;WANG Yuan;NIE Yuanjun;CHENG Bin;ZHAO Jia;CAO Qiufen(Pomology Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;Institute of Agricultural Resources and Economy, Shanxi Academy of Agricultural Sciences, Taiyuan 030006, China;Institute of Agricultural Environment and Resource, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;Biotechnology Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China) |
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| Keywords: | high-throughput sequencing bacterial community diversity chromium resistance Cellulosimicrobium rapid screening |
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