植物-微生物联合修复石油污染土壤的实验研究

筛选高效石油降解菌并考察菌株的石油降解能力,通过植物-微生物联合修复石油污染土壤室内实验,在修复过程中测定了土壤中细菌和固氮菌,碱解氮、速效磷和速效钾的含量变化,同时采用傅立叶变换离子回旋共振质谱(ESI FT-ICR MS)考察了植物-微生物联合修复效果。结果表明,菌株3#、4#的生长适应性较强,其混合菌的降解效果最好,将其混合菌液与植物进行植物-微生物联合修复不同浓度的石油污染土壤,经过150 d的温室降解,最高降解率达到73.47%。ESI FT-ICR MS分析结果表明,与空白组相比,植物组的O1、O2和N1类等化合物相对丰度都发生了显著变化,石油污染物得到一定程度的生物降解。

Experimental study on plant-microbial remediation of oil-contaminated soil

High efficiency degradation bacteria were screened in the laboratory, whose degradation ability was investigated. Plant-microbial remediation of petroleum contaminated soils was conducted, the changes of the number of bacteria and nitrogen-fixing bacteria, the content of N, P, K in the soil were measured in the remediation process, and the remediation effect was also studied. The results showed that the strains of 3# and 4# grew rapidly in the environment, and the degradation effect of their mixed bacteria culture was the best, which was used with plants for the remediation of the simulated oil-contaminated soils. After 150 days of degradation, the highest degradation rate was up to 73.47%. The negative ions ESI FT-ICR MS analysis results showed that O₁,O₂,N₁ class compounds of the plants group changed more significantly compared with the control group, which indicated that the oil in the contaminated soil was biodegraded.