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军曹鱼养殖水体及其肠道异养细菌的耐药性研究
引用本文:王瑞旋,徐力文,王江勇,刘秀明,冯娟.军曹鱼养殖水体及其肠道异养细菌的耐药性研究[J].海洋环境科学,2008,27(6).
作者姓名:王瑞旋  徐力文  王江勇  刘秀明  冯娟
作者单位:1. 中国水产科学研究院南海水产研究所,广东,广州,510300
2. 广东教育学院,广东,广州510300
基金项目:国家863计划项目 , 中国水产科学研究院水产种质资源与养殖技术重点实验室开放基金项目 , 广东省科技攻关项目  
摘    要:选用常用的15种抗生素,通过纸片扩散法(Kirby-Bauer纸片扩散法,简称K-B法),参照NCCLS抗生素敏感试验操作标准,并以金黄色葡萄球菌Staphylococcus aureus ATCC25923和大肠埃希菌Esсheriсhia coli ATCC25922为质控菌株,对周年监测分离自养殖军曹鱼水体及肠道的188株优势菌(包含9个菌属)进行了药物敏感性研究.结果显示,抑菌最强的是环丙沙星(耐药率为0),其次是氯霉素、诺氟沙星、庆大霉素、多粘菌素B、复方新诺明,耐药率均小于40%;抑菌效果最差的依次为羧苄青霉素、青霉素G、氨苄青霉素、痢特灵、利福平,耐药率均在40%以上,其中多重耐药的菌株(对4种或4种以上抗生素产生耐药性)共12株,包括水体菌9株和肠道菌3株.

关 键 词:军曹鱼  养殖水体  肠道  异养细菌  耐药性

Antibiotic resistance of heterotrophic bacteria of digestion guts isolated from cobia(Rachycentron canadum Linnaeus) farming waters
WANG Rui-xuan,XU Li-wen,WANG Jiang-yong,LIU Xiu-ming,FENG Juan.Antibiotic resistance of heterotrophic bacteria of digestion guts isolated from cobia(Rachycentron canadum Linnaeus) farming waters[J].Marine Environmental Science,2008,27(6).
Authors:WANG Rui-xuan  XU Li-wen  WANG Jiang-yong  LIU Xiu-ming  FENG Juan
Institution:WANG Rui-xuan1,XU Li-wen1,WANG Jiang-yong1,LIU Xiu-ming2,FENG Juan1(1.South China Sea Fisheries Research Institute,China Academy of Fishery Science,Guangzhou 510300,China. 2.Guangdong Institute of Education,China)
Abstract:The antibiotic susceptibility tests were carried out by K-B diffusion method on 188 heterotrophic bacteria strains(including 9 genera) isolated for one year both from farming seawater and digestion guts of the cobia.15 different species antibiotics selected were tested to NCCLS performance standards for the antimicrobial susceptibility test,and Staphylococcus aureus ATCC25923 and Escherichia coli ATCC25922 were controlled in the test.The results showed that the most effective antibiotic for the inhibiting g...
Keywords:cobia  farming water  intestine  heterotrophic bacteria  antibiotic-resistance  
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