| 嗜碱细菌复合碳源条件下对麦草木质素的降解 |
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| 引用本文： | 张甲耀,龚利萍,罗宇煊,许威亚,凌颉. 嗜碱细菌复合碳源条件下对麦草木质素的降解[J]. 环境科学, 2002, 23(1): 70-73 |
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| 作者姓名： | 张甲耀 龚利萍 罗宇煊 许威亚 凌颉 |
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| 作者单位： | 1. 武汉大学环境科学系,武汉,430072
2. 武汉大学分析测试中心 |
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| 基金项目： | 国家自然科学基金资助课题(29677012) |
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| 摘 要： | 在碱性液体培养条件下(pH≈10.5), 研究复合碳源共代谢最佳综合条件下嗜碱性木质素降解细菌6号菌株产酶、降解能力及菌株的生长状况.结果显示,虫漆酶(Laccase)在培养的第4天酶活达到最高值2915.37U/L、锰依赖过氧化物酶(MnP)在培养的第8天酶活达到最高值1152.88U/L,培养10d麦草中木质素降解49.84%.同时通过扫描电镜分析探讨了6号菌株降解木质素的微观过程,证明了6号菌株优先降解木质素的特性和降解方式.
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| 关 键 词： | 嗜碱细菌 复合碳源 木质素生物降解 扫描电镜 |
| 文章编号： | 0250-3301(2002)01-04-0070 |
| 收稿时间： | 2001-02-16 |
| 修稿时间： | 2001-02-16 |
Biodegradation of Lignin in Wheat Straw by Alkaliphilic Ligninolytic Bacteria with Compounded Carbons |
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| Zhang Jiayao,Gong Liping,Luo Yuxuan,Xu Weiya and Ling Ji. Biodegradation of Lignin in Wheat Straw by Alkaliphilic Ligninolytic Bacteria with Compounded Carbons[J]. Chinese Journal of Environmental Science, 2002, 23(1): 70-73 |
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| Authors: | Zhang Jiayao Gong Liping Luo Yuxuan Xu Weiya Ling Ji |
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| Affiliation: | Department of Environmental Science, Wuhan University, Wuhan 430072, China. |
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| Abstract: | The article researched enzymes production, the degradation rate of lignin and strain growth of alkaliphilic ligninolytic bacteria strain No. 6 in alkaline liquid medium (pH approximately 10.5) with compounded carbons. The results showed that the highest activities of laccase and MnP were 2915.37 U/L (4th d) and 1152.88 U/L (8th d), respectively, and the strain degraded 49.84% lignin of wheat straw during ten days cultivation. The changes of ultrastructure of wheat straw caused by strain No. 6 attack were investigated with SEM (scan electron microscopy). |
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| Keywords: | alkaliphilic bacteria compounded carbon lignin biodegradation SEM |
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