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海洋耐镉微生物筛选及其生物学特性
引用本文:梁淑君,王雅玲,孙力军,刘颖,徐德峰,励建荣.海洋耐镉微生物筛选及其生物学特性[J].海洋环境科学,2012,31(4):488-491.
作者姓名:梁淑君  王雅玲  孙力军  刘颖  徐德峰  励建荣
作者单位:1. 广东海洋大学食品科技学院,广东湛江,524008
2. 浙江工商大学食品与生物工程学院,浙江杭州310035;渤海大学食品学院,辽宁锦州121031
基金项目:"十二五"国家科技支撑计划,广东海洋大学自然科学面上项目,广东海洋大学大学生创新项目
摘    要:从重金属污染的近海域筛选到一株对镉具有较强抗性和富集能力的微生物Cd20002,该菌株在液体培养基中的最大耐受浓度为1 000 mg/L,在镉浓度达到200 mg/L时,其吸附率高达93.4%,根据形态特征和生理生化鉴定及16S rRNA测序鉴定为蓝杆菌。通过在不同的镉浓度环境对蓝杆菌Cd20002进行培养,测定其生长曲线,并优化菌体生长的条件及其吸附能力。结果表明,蓝杆菌Cd20002既能在重金属镉环境中生存,且富镉能力较强,对水产环境中重金属的生物清排具有重要意义。

关 键 词:重金属污染  蓝杆菌  耐镉  富集能力

Screen and biological characteristics of marine resistance cadmium microbe
LIANG Shu-jun , WANG Ya-ling , SUN Li-jun , LIU Ying , XU De-feng , LI Jian-rong.Screen and biological characteristics of marine resistance cadmium microbe[J].Marine Environmental Science,2012,31(4):488-491.
Authors:LIANG Shu-jun  WANG Ya-ling  SUN Li-jun  LIU Ying  XU De-feng  LI Jian-rong
Institution:2,3(1.College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524008,China;2.College of food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310035,China;3.College of food Science,Bohai University,Jinzhou 121031,China)
Abstract:A energetic bacteria Cd20002 was selected from the shore contaminated by heavy metal,it could stand a high density of 1 000 mg/L in a culture medium.When the density of cadmium was 200 mg/L,its adsorb rate reach a high level of 93.4%.After physiological and biochemical identification and 16S rRNA test,the found Cyanothece sp.Cyanothece Cd20002 were fostered in different cadmium density condition and its growth curve was found.The leaving condition for it and found adsorbing ability were optimized.The result showed that Cyanothece Cd20002 could live at the condition of heavy metal cadmium and can absorb a great deal of the cadmium.It was important to clean the cadmium in aquatic environment by Cyanothece Cd20002 in aquaculture.
Keywords:pollution of heavy metals  Cyanothece  cadmium tolerance  enrichment capacity
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