The suitability of gastropods as biomarkers |
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| Authors: | Harald Meimberg Christiane Schrenk Christian Steinberg Albert Klarenberg Antonius Kettrup |
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| Affiliation: | (1) Institute of Ecological Chemistry, GSF, D-85758 Neuherberg, Germany;(2) Institute of Freshwater and Fish Ecology, Müggelseedamm 310, D-12587 Berlin, Germany;(3) Institute of Zoology, LMU Munich, Karlstr. 23, D-80333 München, Germany |
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| Abstract: | The measurement and induction of mixed function oxyigenases (MFOs) of limnetic gastropods were investigated to estimate their suitability as biomarkers. A determination of MFO activities was performed through the measurement of ethoxyresorufin-O-dealkylase (EROD), pentoxy-(PROD) and benzoxyresorufin-O-dealkylase-activity (BROD). Optimal measuring conditions of these activities were investigated in preparations of the digestive gland of 4 species of limnetic gastropods. Results indicate that MFO-activity inhibiting substances are accumulating in the microsomal pellet, the fraction commonly used for the measurement of MFO-activities. Therefore, the fraction used for induction studies was the postmitochondrial supernatant (PMS). EROD, PROD, and BROD activity ofPlanorbis planorbis andPlanorbis carinatus were measured after a treatment with Aroclor 1254 for 1–17 days. Maximal induction of EROD and PROD were 6 and 10 times the value in the control group ofP. carinatus, respectively induction inP. planorbis was lower. BROD-activity could not been measured inP. planorbis. InP. carinatus, BROD-activity increased to a maximum of 2 times after traetment with Aroclor 1254. The level of induction of EROD-activity is comparable to results described in analog studies with fish. |
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| Keywords: | Aroclor 1254 benzoxyresorufin-O-dealkylase(BROD) biomarkers BROD EROD ethoxyresorufin-O-dealkylase (EROD) gastropods measurement and induction of mixed function oxyigenase (MFO) MFO pentoxyresorufin-O-dealkylase (PROD) PCBs Planorbis carinatus Planorbis planorbis PROD |
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