Detection of Escherichia coli in wastewater based on enzyme immunoassay |
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| Authors: | Haiyan Xi Qiang Cai Miao He Hanchang Shi |
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| Institution: | 1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, China
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| Abstract: | This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horseradish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL-6 × 104 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost. |
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