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| 正交实验选择纤维素酶产生菌的最优综合培养条件 | |
| 引用本文： | 黄蓉姿,万金泉,马邕文,黄明智,杨漪清.正交实验选择纤维素酶产生菌的最优综合培养条件[J].中国环境科学,2012,32(1):130-135. |
| 作者姓名： | 黄蓉姿 万金泉 马邕文 黄明智 杨漪清 |
| 作者单位： | 1. 华南理工大学环境科学与工程学院,广东广州510006;华南理工大学工业聚集区污染控制与生态修复教育部重点实验室,广东广州510006 2. 华南理工大学环境科学与工程学院,广东广州,510006 3. 华南理工大学环境科学与工程学院,广东广州510006;华南理工大学化学与化工学院,广东广州510641 |
| 基金项目： | 广东省首批"节能减排"重大专项，广东省自然科学基金，中央高校基本科研业务费专项资金资助 |
| 摘 要： | 在复合碳源、30℃恒温培养条件下,用正交实验法对影响纤维素酶产生菌降解纤维素的5种单因素培养条件进行了优化,并将优化得到的条件应用于厌氧-好氧废水处理系统.结果表明,MgSO4用量等单因素对纤维素酶活性以及纤维素降解率有不同程度的促进作用;正交实验得到厌氧菌和好氧菌的最佳培养条件不完全一致;在废水处理系统中,5种单因素的最佳综合水平为:30mg/L MgSO4,20mg/L CoCl2,CNP配比为400:5:1,氮源为NH4Cl (28.7mg/L), pH=7.0,此时厌氧菌及好氧菌酶活性分别为4801U/L和4794U/L,酶稳定性分别达到91.0%和95.5%,纤维素降解率为31.9%和28.4% . |
| 关 键 词： | 正交实验 酶活性 酶稳定性 纤维素降解率 |
| 收稿时间： | 2011-04-25; |
Optimization of synthetic culture condition of cellulase producing strains using orthogonal experimental design. |
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| HUANG Rong-zi , WAN Jin-quan , MA Yong-wen , HUANG Ming-zhi , YANG Yi-qing.Optimization of synthetic culture condition of cellulase producing strains using orthogonal experimental design.[J].China Environmental Science,2012,32(1):130-135. | |
| Authors: | HUANG Rong-zi WAN Jin-quan MA Yong-wen HUANG Ming-zhi YANG Yi-qing |
| Institution: | 1(1.College of Environmental Science and Engineering,South China University of Technology,Guangzhou 510006,China;2.Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters,Ministry of Education,South China University of Technology,Guangzhou 510006,China;3.School of Chemistry and Chemical Engineering,South China University of Technology,Guangzhou 510641,China) |
| Abstract: | The five single factors for cellulose biodegradation ability of cellulase producing strains were optimized by orthogonal experiments using mixed carbon source under 30°C,and were used in wastewater treatment system with anaerobic and aerobic processes.Five single culture factors promoted cellulase activity and cellulose degradation-rate in varying degrees.Moreover,the optimal culture conditions of anaerobe obtained through orthogonal experiments were not in accordance with that of aerobe.In the wastewater treatment system,the optimal synthetic condition was MgSO4 of 30mg/L,CoCl2 of 20mg/L,the ratio of CNP of 400:5:1,NH4Cl(of 28.7mg/L) as nitrogen source and pH7.0.The cellulase activities(CA),enzymatic stabilities(ES),and cellulose degradation-rates(CDR) of anaerobe and aerobe were as follows: CA: 4801U/L,4794U/L;ES: 91.0%,95.5%;CDR: 31.9%,28.4%. |
| Keywords: | orthogonal experiment enzyme activity enzymatic stability cellulose degradation rate |
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