C/N比对厌氧颗粒污泥生理生化的影响

研究了进水的初始C/N对厌氧颗粒污泥发酵类型及产甲烷活性的影响.投加钼酸钠抑制产甲烷菌的一组实验主要研究产酸阶段C/N比对厌氧颗粒污泥的作用,发现初始C/N为12、56、156时,形成的是丁酸型发酵;当初始C/N为200时,可实现乙醇型发酵.不同的C/N比对产酸阶段微生物的胞外聚合物也有一定的影响.随着C/N比的减少,胞外聚合物的总量、胞外多糖以及胞外蛋白质都呈现先增大后减少的趋势,C/N比为56时达到最大值.未加钼酸钠的一组实验研究了C/N比对产甲烷活性的影响,发现C/N比为200时有最高的COD去除率和产甲烷活性;随着C/N比的减小胞外聚合物的总量先减少后增加,厌氧颗粒污泥胞外聚合物在C/N比为200时总量达到最大值.在C/N比为156时胞外蛋白质达到最大值.通过红外光谱发现,C/N比对厌氧颗粒污泥表面基团也有一定的影响,C/N比为200时,出现1350~1260cm-1波段的峰.其他3种C/N比下无该波段的峰.

Effects of C /N ratio on physiological biochemical characteristics of anaerobic granular sludge

Eeffects of initial influent carbon-nitrogen ratio on fermentative types and methanogenic activity of anaerobic granular sludge were studied using the group of experiment which adding molybdate to restrain the activity of methanogens to study the effect of C/N ratio on anaerobic granular sludge during the acidification period,when the initial C/N ratio was 12,56,156,the type of fermentation was butyric acid type fermentation and when the initial C/N ratio was 200,forming ethanol type fermentation.Different C/N ratio had influence on EPS of anaerobic granular sludge during acidification period.With the C/N ratio decreased,the total amount of EPS,extracellular polysaccharide and extracellular proteins had shown a decreasing trend after the first increase,reaching maximum when C/N ratio was 56.Using the group of experiment which did not add molybdate to study the effect of methanogenic activity of anaerobic granular sludge,it had the highest COD removal efficiency and methanogenic activity when C/N ratio was 200.With the C/N ratio decreased,the total amount of EPS had shown a increasing trend after the first decrease.The total amount of EPS reached maximum when C/N ratio was 200 and the extracellular proteins reached maximum when C/N ratio was 156.The analysis of functional group of surface of anaerobic granular sludge with Fourier transform infrared dspectrometry showed when C/N ratio was 200,there was 1350~1260 band peak.The other three C/N ratio had no 1350~1260 band peaks.