| 费氏中华根瘤菌ntrBC基因突变株的构建及其特性 |
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| 引用本文： | 龚焘,陆祖军,陈钢,曹永强,武波.费氏中华根瘤菌ntrBC基因突变株的构建及其特性[J].应用与环境生物学报,2007,13(4):523-525. |
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| 作者姓名： | 龚焘 陆祖军 陈钢 曹永强 武波 |
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| 作者单位： | 广西大学生命科学与技术学院微生物及植物遗传工程教育部重点实验室,广西亚热带生物资源保护利用重点实验室,南宁,530005 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金 |
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| 摘 要： | 从费氏中华根瘤菌(Sinorhizobium fredii)HN01基因文库的重组质粒pGXHN300上获得ntrB、ntrC基因完整序列.通过自杀质粒pK18mob同源单交换的方法,分别获得了HN01菌株的ntrB和ntrC基因的突变株GXHNB、GXHNC.功能检测证明GXHNB、GXHNC能以硝酸盐为唯一氮源进行正常生长,但不能以铵源作为唯一氮源进行正常生长.图4表2参15
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| 关 键 词： | 费氏中华根瘤菌 ntrB基因 ntrC基因 突变体 |
| 收稿时间： | 2006-05-08 |
| 修稿时间： | 2006-09-05 |
Construction and Characterization of ntrB- and ntrC- Mutants of Sinorhizobium fredii |
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| GONG Tao,LU Zujun,CHEN Gang,CAO Yongqiang,WU Bo.Construction and Characterization of ntrB- and ntrC- Mutants of Sinorhizobium fredii[J].Chinese Journal of Applied and Environmental Biology,2007,13(4):523-525. |
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| Authors: | GONG Tao LU Zujun CHEN Gang CAO Yongqiang WU Bo |
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| Institution: | Key Laboratory of Microbial and Plant Genetic Engineering of Ministry of Education, Key Laboratory of Subtropical Bio-resource Conservation and Utilization of Guangxi, College of Life Science and Technology, Guangxi University, Nanning 530005, China |
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| Abstract: | The ntrB and ntrC genes of Sinorhizobium fredii strain HN01 were obtained from recombinant plasmid pGXHN300. The ntrB-and ntrC-mutants, named GXHNB and GXHNC, were constructed through homologous recombination caused by a single crossover event. Compared with parent strain HN01, the GXHNB and GXHNC were able to grow on the medium with nitrate as the sole nitrogen source, but grew weakly on the medium with ammonium as the sole nitrogen source. Fig 4, Tab 1, Ref 15 |
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| Keywords: | Sinorhizobium fredii ntrB ntrC mutant |
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