Quantification and Genotyping of Aichi Virus 1 in Water Samples in the Kathmandu Valley,Nepal |
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| Authors: | Eiji Haramoto Masaaki Kitajima |
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| Affiliation: | 1.Interdisciplinary Center for River Basin Environment, Graduate Faculty of Interdisciplinary Research,University of Yamanashi,Kofu,Japan;2.Division of Environmental Engineering, Faculty of Engineering,Hokkaido University,Sapporo,Japan |
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| Abstract: | Aichi virus 1 genomes were detected by quantitative PCR in groundwater from shallow dug (10/22) and tube wells (1/15), river water (14/14), and sewage (1/1), with the maximum concentration of 4.0 × 109 copies/l. Nucleotide sequencing analysis demonstrated the prevalence of genotype B in the virus positive samples. |
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