海洋费氏弧菌培养条件的研究

以本室保存的一株海洋费氏弧菌作为实验对象,采用单因素试验方法研究培养基起始pH值、盐浓度和摇瓶装液量等因素对菌株生长的影响,利用分光光度法测定其生长曲线,采用均匀设计方法,对该菌的培养基组分进行优化研究,确定其适合的生长条件,结果表明:当培养温度为30℃,通气量为180r/min时,该菌株在20h左右达到生长稳定期,对甘油的利用率要优于葡萄糖,盐浓度在5%以下时生长良好,最适装液量为60mL/250mL,培养液初始pH值为8.0。确定100mL培养基配方为:蛋白胨2g,酵母粉1.0g,氯化铵1.2g,氯化钠0.2g,磷酸二氢钾1.0g,此条件下可以得到最大的生物量。

Culture Conditions of Vibrio fischer

Influences of initial pH,salinity and volume on Vibrio fischer were studied by single factor test respectively.Value of growth curve was drawn through spectrophotometer.Uniform design was used to confirm medium elements.Strain reached the growth of stable phase after 20h under the condition of rotating speed 180r/min and culture temperature 30℃.Glycerol was better carbon source than glucose and results showed that the strain was fit for the culture conditions of salinity no more than 5% ,filling volume 60mL per flask 250mL,and initial pH 8.0.The optimal culture medium elements in 100mLwere obtained by uniform design as peptone 2g,yeast 1.0g,NH4Cl 1.2g,NaCl 0.2g and KH2PO4 1.0g.