类球红细菌利用混合底物光合产氢特性研究

研究了类球红细菌(Rhodobacter sphaeroides)利用有机酸和糖类作为混合底物的光合产氢特性。初步研究了6种有机酸和3种糖类的产氢活性,并选用具有最大底物转化效率的琥珀酸钠和最大平均产氢速率的葡萄糖混合进行光合产氢,对底物利用和pH变化对产氢影响进行了探索。结果表明,类球红细菌利用混合底物可提高平均产氢速率、底物转化效率和底物利用率,琥珀酸钠和葡萄糖按15/15(mmol/L)混合最大平均产氢速率达19.1mL/(L·h),底物转化效率和底物利用率分别提高了35.3%和13.3%。混合底物利用中的pH值调节和底物利用率提高是有利于类球红细菌的氢气产生提高的重要因素。

Characteristics of Hydrogen Photoproduction by Rhodobacter sphaeroides Using Mixed Substrates

Characteristics of hydrogen photoproduction by Rhodobacter sphaeroides using organic acids and sugars as mixed substrates were studied.Six kinds of organic acids and three kinds of sugars were experimented,and sodium succinate with maximum substrate conversion efficiency and glucose with maximum average hydrogen production rate were selected as mixed substrates for photoproduction of hydrogen.The effects of substrate utilization and pH changes on hydrogen production were discussed.Results showed that the mixed substrates can improve average hydrogen production rate,substrate conversion efficiency and substrate utilization rate,relative to hydrogen production from single carbon source.The maximum average hydrogen production rate reached to 19.1mL/(L·h) from sodium succinate and glucose mixed by 15:15(mmol/L),and substrate conversion efficiency and substrate utilization rate were also increased by 35.3% and 13.3%.Adjustment of pH value and improvement of substrate utilization rate of mixed substrate were considered as main factors for increase of hydrogen production efficiency.