Protein–Surfactant interactions in the defensive skin secretion of the Red Sea trunkfish Ostracion cubicus |
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Authors: | E Kalmanzon E Zlotkin R Aknin-Herrmann |
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Institution: | (1) Department of Animal and Cell Biology, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel, and The Interuniversity Institute for Marine Sciences, Eilat 88103, Israel, IL |
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Abstract: | With the aid of column chromatography, solvent fractionation and toxicity assays we made the following observations. (1)
The defensive skin secretion of the Red Sea trunkfish Ostracion cubicus consists, on a dry weight basis, of about 15% proteins, of 5 to 70 kDa molecular weight. (2) The proteins serve as an integral
part of the chemical defense mechanism and fulfill two essential roles: (a) as ichthyotoxins which affect fish through external
application into the surrounding water; and (b) as modifiers/regulators of the action of the well-known active ingredient
of trunkfish secretion, the cationic, quaternary-ammonium, surfactant pahutoxin (PHN). The latter, when isolated from other
components, plays only a limited role in ichthyotoxicity. (3) The regulatory role is performed by nontoxic, and as yet unidentified,
proteins which potentiate PHN ichthyotoxicity. Potentiation is presumably preceded and mediated by the protein–PHN association,
suggesting a guiding “pharmacokinetic” role of the regulatory proteins. (4) Finally, it may be concluded that the entire secretion
process functions with unique pharmacological complexity due to the multiplicity of active substances and their interdependency.
Received: 7 May 1998 / Accepted: 7 June 1999 |
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