| Ni2+对降解2-氯酚厌氧系统古细菌种群结构的影响 |
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| 引用本文： | 黄爱群,陈 皓,陈 玲,戴亚蕾.Ni2+对降解2-氯酚厌氧系统古细菌种群结构的影响[J].环境科学研究,2007,20(6):94-100. |
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| 作者姓名： | 黄爱群 陈 皓 陈 玲 戴亚蕾 |
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| 作者单位： | 1. 同济大学,环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海,200092
2. 同济大学,医学院,免疫实验室,上海,200092 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 提取降解2-氯酚(2-chlorophenol,2-CP)的厌氧颗粒污泥在Ni2 冲击前后样品的总DNA,用古细菌特异性引物ARC21F/ARC958R进行16S rDNA的聚合酶链式反应(Polymerase Chin Reaction,PCR)、克隆、测序及序列分析等,研究了Ni2 冲击及恢复性驯化对2-CP驯化后厌氧颗粒污泥中古细菌种群多样性的影响.结果表明:Ni2 冲击对古细菌种群结构影响较大,冲击前后厌氧颗粒污泥中存在共有的古细菌菌种(Methanothrix soehngenii,Methanosaeta concilii及Uncultured archaeon等).冲击后厌氧污泥中新出现的古细菌有Toluene-degrading Methanogenic consortium archaeon M1及Candidatus Methanoregula boonei strain 6A8等.经过10 d的恢复性驯化后,厌氧污泥中又出现了新的古细菌Methanobacterium subterraneum及Methanobacterium palustre 等.2-CP厌氧降解系统经过Ni2 冲击后,古细菌种群多样性显著增加,系统能较快恢复对2-CP的降解能力.
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| 关 键 词： | 2-氯酚 Ni2 冲击 厌氧颗粒污泥 克隆 古细菌 降解能力 氯酚 厌氧降解 系统 细菌种群结构 结构影响 Effects Degradation Anaerobic Granular Sludge 恢复性 strain 新出现 厌氧污泥 存在 结果 种群多样性 驯化 研究 序列分析 测序 |
| 文章编号： | 1001-6929（2007）06-0094-07 |
| 收稿时间： | 2007-03-19 |
| 修稿时间： | 2007-06-05 |
Effects of Ni2+ on Archaea Community Structure in Anaerobic Granular Sludge for 2-Chlorophenol Degradation |
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| HUANG Ai-qun,CHEN Hao,CHEN Ling and DAI Ya-lei.Effects of Ni2+ on Archaea Community Structure in Anaerobic Granular Sludge for 2-Chlorophenol Degradation[J].Research of Environmental Sciences,2007,20(6):94-100. |
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| Authors: | HUANG Ai-qun CHEN Hao CHEN Ling and DAI Ya-lei |
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| Institution: | State Key Laboratory of Pollution Control and Resource Reuse, College ofEnvironmental Science and Engineering, Tongji University, Shanghai 200092, China;State Key Laboratory of Pollution Control and Resource Reuse, College ofEnvironmental Science and Engineering, Tongji University, Shanghai 200092, China;State Key Laboratory of Pollution Control and Resource Reuse, College ofEnvironmental Science and Engineering, Tongji University, Shanghai 200092, China;Department of Immunology, School of Basic Medical Science, Tongji University, Shanghai 200092, China |
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| Abstract: | The effects of Ni2+ on Archaea community structure in anaerobic granular sludge for 2-chlorophenol degradation were analyzed by 16S rDNA-based approach. Total DNA was extracted directly from the 2-CP-acclimated anaerobic sludge,the sludge being shocked by Ni2+ and the sludge being recovered after shockrespectively, and then amplified by polymerase chain reaction (PCR) technique with the specific primer pair ARC21F/ARC958R for Archaea. The positive PCR productswere cloned and sequenced. The sequences analysis shows that Archaea community structure shifts obviously after being shocked by Ni2+, and there exist some common Archaea among these sludge, including Methanothrix soehngenii, Methanosaeta concilii and Uncultured archaeon, etc. Some special Archaea are found after being shocked by Ni2+, such as Toluene-degrading Methanogenic consortium archaeon M1 andCandidatus Methanoregula boonei strain 6A8, etc. Compared with the sludge beingshocked by Ni2+, some special Archaea appear in the sludge being recovered for ten days, including Methanobacterium subterraneum and Methanobacterium palustre,etc. After being shocked by Ni2+, Archaea community diversity increases and the anaerobic system could recover the ability for 2-CP-degradation quickly. |
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| Keywords: | 2-CP shock by Ni2+ anaerobic granular sludge cloning Archaea |
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