Incubation temperature has long-term effects on behaviour of young Pine snakes (Pituophis melanoleucus)

Summary Eggs of pine snakes (Pituophis melanoleucus) were incubated at constant temperatures of 21°, 23°, 26°, 28°, 30° and 32° C to determine behavioural differences as a function of incubation temperature. For all behavioural and physiological measures hatchlings from medium incubation temperatures (26°, 28°) performed tests better than those hatchlings from eggs incubated at low temperatures (21°, 23°). For some behavioural tests hatchlings from eggs incubated at high temperature (30°, 32°) performed less well than medium temperature hatchlings. These differences were not due to ambient temperatures or age of testing since these were held constant. Some of the behavioural differences persisted for 24 weeks.