Production of Curdlan Grown on Cassava Starch Waste Hydrolysates |
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| Authors: | Shengjun Wu Yan Xu " target="_blank">Shujun Wang |
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| Institution: | 1.Jiangsu Marine Resources Development Research Institute,Lianyungang,China;2.School of Marine Science and Technology,Huaihai Institute of Technology,Xinpu,China;3.Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening,Huaihai Institute of Technology,Lianyungang,China;4.Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Huaihai Institute of Technology,Lianyungang,China |
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| Abstract: | Cassava starch waste hydrolysates (CSWHs) with different degrees of polymerisation, i.e., CSWHs-1, CSWHs-2 and CSWHs-3, were prepared through the hydrolysis of cassava starch waste with thermostable a-amylase from Thermococcus sp. HJ21. The prepared CSWHs were then used as a carbon source for curdlan production with Alcaligenes faecalis ATCC 31749. The amount of curdlan produced and the glucosyltransferase activity during curdlan synthesis increased more obviously when CSWHs-2 was used as the carbon source than when glucose was used. Using both carbon sources, the maximum curdlan production was observed at day 5, and the maximum glucosyltransferase activity was observed at day 4. Glucosyltransferase activity decreased thereafter, and biomass continued to increase until the end of the experiment (day 6). Results indicated that the enhanced curdlan production with CSWHs as the carbon source was highly correlated with glucosyltransferase activity. |
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