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水库沉积物贫营养型好氧反硝化菌(Pseudomonas sp.)分离及其对微污染水体脱氮效率分析
引用本文:王慎,张思思,许尤,官卓宇,杨正健,刘德富,马骏. 水库沉积物贫营养型好氧反硝化菌(Pseudomonas sp.)分离及其对微污染水体脱氮效率分析[J]. 环境科学学报, 2020, 40(3): 915-921. DOI: 10.13671/j.hjkxxb.2019.0418
作者姓名:王慎  张思思  许尤  官卓宇  杨正健  刘德富  马骏
作者单位:湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068,湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068,湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068,湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068,三峡大学,三峡库区生态环境教育部工程研究中心,宜昌443002,湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068;三峡大学,三峡库区生态环境教育部工程研究中心,宜昌443002,湖北工业大学土木建筑与环境学院,河湖生态修复与藻类利用湖北省重点实验室,武汉430068
基金项目:国家自然科学基金项目(No.91647207,51879099,51779128)
摘    要:为探究深水水库沉积物微生物功能特征及利用价值,于2019年在实验室对小湾水库表层沉积物微生物进行了驯化分离,并分析了其中一株细菌的脱氮效率.结果表明,分离出的细菌XW731经鉴定属于假单胞菌属(Pseudomonas sp.),是一种贫营养型好氧反硝化菌;在分别以NH4+-N、NO3--N和NO2--N为唯一氮源时,该菌对NH4+-N、NO3--N和NO2--N去除率分别为33.6%、68.5%和9.1%;以NH4+-N和NO3--N为氮源时,对NH4+-N和NO3--N去除率分别为66.4%、89.6%,同步硝化反硝化能力更强.将该菌投加到两种城市微污染水体后测试表明,该菌对城市河道水体的NH4+-N和NO3--N去除率分别为38.3%和42.4%,对城市降雨水体的NH4+-N和NO3--N去除率分别为22.2%和7.7%.

关 键 词:好氧反硝化菌  异养硝化  脱氮特性
收稿时间:2019-09-15
修稿时间:2019-10-24

Isolation of aerobic denitrifying bacteria(Pseudomonas sp.) from reservoir sediments and analysis of its denitrification efficiency in micro-polluted water
WANG Shen,ZHANG Sisi,XU You,GUAN Zhuoyu,YANG Zhengjian,LIU Defu and MA Jun. Isolation of aerobic denitrifying bacteria(Pseudomonas sp.) from reservoir sediments and analysis of its denitrification efficiency in micro-polluted water[J]. Acta Scientiae Circumstantiae, 2020, 40(3): 915-921. DOI: 10.13671/j.hjkxxb.2019.0418
Authors:WANG Shen  ZHANG Sisi  XU You  GUAN Zhuoyu  YANG Zhengjian  LIU Defu  MA Jun
Affiliation:Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068,Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068,Engineering Research Center of Ecoenvironment in Three Gorges Reservoir Region, Ministry of Education, Three Gorges University, Yichang 443002,1. Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068;2. Engineering Research Center of Ecoenvironment in Three Gorges Reservoir Region, Ministry of Education, Three Gorges University, Yichang 443002 and Hubei Key Laboratory of Ecological Restoration of River-lakes and Algal Utilization, School of Civil Engineering, Architecture and Environment, Hubei University of Technology, Wuhan 430068
Abstract:In order to study the characteristics and application of microorganisms in sediments of deep-water reservoir, the microorganisms in surface sediments of the Xiaowan reservoir were cultivated and isolated in the laboratory, and the denitrification efficiency of one isolate was analyzed. The results show that the isolate, XW731, is a type of oligotrophic aerobic denitrifying bacteria, which belongs to Pseudomonas. When NH4+-N, NO3--N and NO2--N was the sole nitrogen source, respectively, the removal rates of NH4+-N, NO3--N and NO2--N were 33.6%, 68.5% and 9.1%. when NH4+-N, NO3--N were nitrogen sources, the removal rates of NH4+-N, NO3--N were 66.4%, 89.6%, meanwhile, simultaneous nitrification and denitrification ability were stronger. Furthermore, the bacteria were added to two kinds of micro-polluted water, XW731 had certain denitrification effect and the removal rates of NH4+-N, NO3--N were 38.3%, 42.4% respectively in urban river water, and 22.2%, 7.7% respectively in urban rainfall water.
Keywords:aerobic denitrifier  heterotrophic nitrification  nitrogen removal characteristics
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