固定化真菌漆酶对分散兰-2BLN的脱色和降解

采用改良壳聚糖固定化的真菌漆酶对染料分散兰-2BLN进行脱色和降解条件的研究，探索了固定化漆酶活力、处理时间、染料浓度、温度和pH对其降解效果的影响。结果表明，固定化漆酶脱色降解分散兰-2BLN的适宜条件为：固定化漆酶活力18.2U／mL，染料浓度100mg／L，温度40℃，pH4.6，在上述条件下降解1.5h，分散兰-2BLN脱色率能达到87.68％。重复分批使用固定化漆酶处理2BLN兰，在使用6批次后，脱色率仍能保持在55％以上，其催化效率得到了较大提高。

Study on Decolorization and Degradation of Disperse Blue-2BLN by Immobilized Fungle Laccase

In this paper, the conditions for deeolorization and degradation of Disperse Blue-2BLN by laccase immobilized on the improved chitosan were studied in the fields of activity of immobilized laccase, process time, concentration of Disperse Blue-2BLN, temperature and pH. The results showed that the optimum conditions for degrading Disperse Blue-2BLN by immobilized laccase were as follows： 18.2U/mL for the activity of immobilized laccase, 100mg/L for the concentration of Disperse Blue-2BLN, 40℃ for the reaction temperature and pH 4.6. Keeping the optimum conditions above for 1.5h, the decolorization rate of Disperse Blue-2BLN could reach 87.68%. The usage of immobilized laccase to treat Disperse Blue-2BLN was repeated in batch. It showed that after six repetitions of operation the decolofization rate was still higher than 55%. Thus the catalytic efficiency of laccase was greatly improved.