Kinships in a natural meadow vole population

Summary We have devised a radionuclide technique that allows the assessment of matrilineal kinship by the transfer of unique combinations of gamma-emitting radionuclides from pregnant and lactating female meadow voles (Microtus pennsylvanicus) to their offspring. A pool of prospective fathers can be established from field records of reproductively active males that were alive at the time of conception of the female's litter. Electrophoresis of 6 proteins allowed us to eliminate genetically incompatible males from the prospective pool of fathers. A log-likelihood statistic provided an estimate of the probability of paternity. In the case of numerical ties physical proximity of the males to the mother at the time of conception was used. With these techniques, we established 40 kinships in a field population of meadow voles.