高浓度苯酚降解产电菌ZY07的筛选及其降解产电特性

从以苯酚为燃料且运行至稳定的微生物燃料电池阳极碳毡上筛选驯化获得一株能够降解高浓度苯酚的产电菌株ZY07，经18S rRNA序列分析，鉴定该菌为热带假丝酵母菌(Candida tropicalis)。初步探究了菌株ZY07的生物学特性和产电特性。结果表明，经驯化后，菌株ZY07的耐酚质量浓度可达到2 000 mg·L⁻¹，48 h基本能完全降解1 700 mg·L⁻¹的苯酚；菌株ZY07的最适生长及降酚条件为：接菌量为8%、pH为8、温度为35 ℃。循环伏安分析结果表明，菌株ZY07具有良好的电化学活性，以ZY07构建的MFC最大输出电压为0.72V，最大功率密度达48.02 mW·m⁻²；阳极碳毡扫描电镜显示，产电菌ZY07附着在碳毡表面形成生物膜。综合循环伏安和扫描电镜分析结果可推测，菌株ZY07是通过生物膜与电极表面直接接触的方式传递电子。

Screening of high-concentration phenol-degrading electrogenic strain ZY07 and its biodegradation and electricity-generating characteristics

An electrogenic strain ZY07 capable of degradation high concentration phenol was screened from the anodic carbon felt in a stable running microbial fuel cells with phenol as fuel and domesticated, 18S rRNA analysis showed that the bacteria was the Candida tropicalis. The biological characteristics and electrical production properties of the strain ZY07 were preliminarily investigated. The results showed that the phenol resistant concentration of strain ZY07 could reach 2 000 mg·L−1, and this strain could almost completely degrade 1 700 mg·L−1 in 48 h after domestication; The best growth and phenol-degrading conditions of strain ZY07 were following: the receiving volume of 8% , initial pH 8 and 35 ℃; cyclic voltammogram analysis showed that strain ZY07 had a good electrochemical activity, the maximum voltage output of MFC built by ZY07 was 0.72V, and the maximum power density could reach 48.02 mW·m−2; the scanning electric microscope of anode carbon felt showed that ZY07 attached to the carbon felt surface to form biofilms; based on the cyclic voltammogram and SEM results, it is speculated that the strain ZY07 transmits the electrons through the direct contact between the biofilm and the electrode surface.