烟曲霉菌吸附染料的解吸及其机理

研究了烟曲霉(Aspergillus fumigatus)生长菌体吸附染料后的解吸情况及其机理。结果表明,吸附有直接染料或活性染料的生长菌体在甲醇体系中均有相当高的解析率;解吸剂溶液的酸碱性对解吸率有很大影响,酸性环境下解吸受到抑制,而碱性环境可显著提高解吸速度和解吸率;初始温度对解吸的影响较小,温度升高,解吸率有所降低;解吸剂用量较少,只需少量解吸剂,吸附在生长菌体中的染料就可比较彻底的解吸出来。采用甲醇解吸吸附有活性艳蓝KN-R的生长菌体的作用机理为,甲醇和染料分子中的磺酸基发生了放热反应,生成了磺酸甲酯。吸附有活性艳蓝KN-R的生长菌体在甲醇体系中的解吸符合一级指数衰减模型,解吸初始阶段,反应速度较快;反应中后期,甲醇要突破缠绕致密的菌丝球和生物膜的障碍才能和染料相接触,因而反应速度较慢。吸附有染料的生长菌体在采用甲醇解吸出吸附在其上的染料后,仍可接入到染料体系中对其进行吸附脱色,且可连续使用3次以上。

Desorption and Mechanism of Dye Adsorbed Growing Mycelia of Aspergillus fumigatus

Characteristics and mechanism on desorbing of the dye adsorbed growing mycelia of Aspergillus fumigatus were studied. Results showed that the desorptive rate of direct or reactive dye adsorbed growing mycelia was relatively high. pH value of desorbent had remarkable effect on desorptive rate, and the desorption rate was restrained on acidic circumstance while improved on the base circumstance. Desorption rate reduced appropriately when initial temperature rose. Dye adsorbed growing mycelia could be easily desorbed in existence of a few desorbent. The desorption of reactive brilliant blue adsorbed growing mycelia by methanol was in accordance with the first exponential decay model, with reactive rate comparatively rapid on the initial phase and slow on the middle or behind because of obstacle of compact mycelium. After the dye absorbed growing mycelia was desorbed, the mycelia still could be used for three times.