鄱阳湖南矶山自然保护区鲤、鲫的随机扩增多态DNA分析

利用RAPD（随机扩增多态）标记分析湖内鲤、鲫群体遗传多样性，从40个随机引物中各筛选出8个引物适合鲤、鲫群体RAPD扩增。在鲤群体中，共检测出60条带，其中多态性带42条，多态位点比率为70.00%；而在鲫群体中，共检测出61条带，其中多态性带40条，多态位点比率为65.57%。用POPGENE软件分析实验数据，结果显示：湖内鲤群体的遗传多样性水平(He=0.230 1，H0=0.391 0)和鲫群体的遗传多样性水平(He=0.218 6，H0=0.375 8)都较高，都有较大的遗传变异。而在鲤、鲫群体内，雌性群体与雄性群体相比较，其各自的遗传多样性很接近。

RAPD ANALYSIS OF GENETIC DIVERSITY OF CYPRINUS CARPIO AND CARASSIUS AURATUS IN NANJISHAN NATURE RESERVE IN POYANGHU LAKE

The colonial genetic variation in Cyprinus carpio and Carassius auratus was analyzed with RAPD technique. Eight primers were screened out from 40 random primers on the genomic DNA in Cyprinus carpio. A total of 60 bands were generated, of which polymorphic bands were 42, with the percentage of polymorphism being 70.00%. Using the 8 primers for genomic DNA of Carassius auratus, 61 bands were generated, of which 40 were polymorphic, with the percentage of polymorphism being 65.57%. As analyzed by software POPGENE, the colonial genetic diversity was both high in Cyprinus carpio (He=0.230 1, H0=0.391 0) and Carassius auratus (He=0.218 6, H0=0.375 8). However, in the population of Cyprinus carpio and Carassius auratus, the colonial genetic diversity was very similar in females and males.