IC反应器处理啤酒废水的效能及其微生物群落动态分析

在35℃下连续运行内循环厌氧反应器(IC),通过不断提高进水容积负荷,研究不同运行负荷下反应器处理模拟啤酒废水的运行情况,并探讨微生物的种群结构与其活性变化之间的相互关系.IC反应器效能分析表明其最大进水容积负荷(以COD计)可达20 kg.(m3.d)-1,COD去除率为85%以上,最大比产甲烷活性(以VSS计)可达210 mL.(g.d)-1;污泥脱氢酶活性与细菌DGGE图谱表明反应器中脱氢酶活性的变化趋势与细菌DGGE条带总光强度的变化趋势一致,DGGE图谱中细菌条带的总光密度值可作为厌氧体系中生物量的一个参考指标;辅酶F420与古细菌DGGE分析表明,污泥中辅酶F420含量与甲烷鬃菌属(Methanosaeta sp.)的相对丰度有一定的相互联系,在低负荷条件下甲烷八叠球菌属(Methanosarcina mazei)优势地位比较显著,随着负荷的提高,甲烷鬃菌属(Methanosaeta sp.)优势地位逐渐显著,且辅酶F420含量随之升高,当最大负荷为20kg.(m3.d)-1时,辅酶F420的含量(以VSS计)达到0.16μmol.g-1,而此时甲烷鬃菌属(Methanosaeta sp.)占据尤为明显的优势地位;聚类分析(UPGAMA)和Shannon指数也表明在不同反应负荷下,微生物的群落结构变化明显.

Performance and Microbial Community Dynamic Characteristics of an Internal Circulation Reactor Treating Brewery Wastewater

A lab-scale internal circulation reactor(IC) fed by artificial brewery wastewater was operated with increasing volumetric loading rate under 35℃continuously.The reactor performance and the relationship between microbial community structure and bioactivity in the anaerobic sludge were investigated during the operation.The COD removal efficiency was above 85%,furthermore,the maximum volumetric loading rate(VLR) and the maximum specific methanogenic activity(SMA) of the reactor could be up to 20 kg·(m3·d)-1 and 210 mL·(g·d)-1 respectively.The results from the dehydrogenase and the bacteria DGGE experiments demonstrated that the dehydrogenase variation tendency was positively correlated to total light intensity of the whole bacteria DGGE bands for each sample.The total light intensity of the whole bacteria DGGE bands can use as a referential index for biomass liveweight in anaerobic system.Moreover,the coenzyme F420 content related to the relative abundance of Methanosaeta based on coenzyme F420and archaebacteria DGGE analysis.As the volumetric loading rate increased,Methanosaeta became significantly dominant,which was accompanied by the coenzyme F420 content increasing.The content could be up to 0.16 μmol·g-1,meanwhile,the superiority of Methanosaeta became significantly obvious;UPGAMA analysis and Shannon index also confirmed the dynamic changes of microbial community structure during the operation.