凹凸棒石粘土固定辣根过氧化物酶处理含酚废水

采用凹凸棒石粘土、可溶性淀粉和工业水玻璃作为制备凹凸棒石粘土基颗粒的材料,并将制备的凹凸棒石粘土基颗粒进行改性,得到了改性后的凹凸棒石基多孔材料,并将其作为固定辣根过氧化物酶的载体,再应用于含酚废水的处理研究,取得了良好的效果。实验结果表明,辣根过氧化物酶的最佳固定化条件为:单位酶活载体量1 mg、固定化时间1.5小时、固定pH值5。并且在固定化酶循环使用6次后,苯酚去除率仍能达到62.3%。

Horseradish peroxidase immobilization in palygorskite-based material for phenol wastewater treatment

Palygorskite clay, soluble starch and commercial silicate can be used to prepare a type of palygorskite-based granulated material. The granulated material was modified by modified palygorskite-based porous materials. The excellent performance of the porous materials applied in phenol wastewater removal was proved after horseradish peroxidase were fixed on the materials. The experimental results show that 1 mg unit volume of activity vector fixed time of 1.5 hours and pH value of 5 will ensure the best immobilization condition of horseradish peroxidase. After 6 times recycle test of the immobilized enzyme, phenol removal rate can reach 62.3%.