|
|||||
|
|
| PCR-DGGE研究亚硝化-电化学生物反硝化全自养脱氮工艺细菌多样性 | |
| 引用本文： | 柳栋升,王海燕,杨慧芬,伊静,周岳溪,张娜,庞朝辉.PCR-DGGE研究亚硝化-电化学生物反硝化全自养脱氮工艺细菌多样性[J].环境工程学报,2012,6(9):3349-3355. |
| 作者姓名： | 柳栋升 王海燕 杨慧芬 伊静 周岳溪 张娜 庞朝辉 |
| 作者单位： | 1. 中国环境科学研究院水污染控制技术研究室,环境基准与风险评估国家重点实验室,北京100012 北京科技大学土木与环境工程学院,北京100083 2. 中国环境科学研究院水污染控制技术研究室,环境基准与风险评估国家重点实验室,北京100012 3. 北京科技大学土木与环境工程学院,北京,100083 4. 中国矿业大学北京,北京,100083 |
| 基金项目： | 国家自然科学基金资助项目(50708120);甘肃省创新团队项目;国家"863"高技术研究发展计划项目(2004AA649320) |
| 摘 要： | 采用分子生物学手段PCR-DGGE技术对亚硝化-电化学生物反硝化全自养脱氮工艺细菌的多样性进行了研究。结果表明,亚硝化段内主要的细菌种群为相似于Nitrosomonas sp.(AJ224410)和Nitrosomonas sp.NM41(AF272421)的种群,相似性分别为97%和94%;电化学生物反硝化段细菌类群主要有β-proteobacteria类群、γ-proteobacteria类群和Chlo-roflexi类群。填料上生物膜细菌种群较底部泥水混合物丰富,两者细菌种群相似性为75%;底部泥水混合物样中存在与厌氧氨氧化菌Brocadia anammoxidans(AF375994)相似性为93%的菌种,而填料上生物膜中存在与Thioalkalivibrio sp.K90mix(EU709865)和Thiobacillus thioparus(AJ243144)相似性分别为94%、97%的菌种,其中Thiobacillus thioparus(AJ243144)是典型的硫自养反硝化菌,表明填料上生物膜中有大量的硫自养反硝化菌。 |
| 关 键 词： | PCR-DGGE 脱氮 亚硝化-电化学生物反硝化 细菌多样性 |
Bacterial diversity of complete autotrophic nitrite-nitrification and electrochemical bio-denitrification combined process using PCR-DGGE |
|
| Liu Dongsheng,Wang Haiyan,Yang Huifen,Yi Jing,Zhou Yuexi,Zhang Na and Pang Zhaohui.Bacterial diversity of complete autotrophic nitrite-nitrification and electrochemical bio-denitrification combined process using PCR-DGGE[J].Techniques and Equipment for Environmental Pollution Control,2012,6(9):3349-3355. | |
| Authors: | Liu Dongsheng Wang Haiyan Yang Huifen Yi Jing Zhou Yuexi Zhang Na and Pang Zhaohui |
| Institution: | Department of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, State Key Laboratory of Environmental Criteria and Risk Assessment, Beijing 100012, China;Civil and Environment Engineering School, University of Science and Technology Beijing, Beijing 100083, China;Department of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, State Key Laboratory of Environmental Criteria and Risk Assessment, Beijing 100012, China;Civil and Environment Engineering School, University of Science and Technology Beijing, Beijing 100083, China;China University of Mining & Technology, Beijing 100083, China;Department of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, State Key Laboratory of Environmental Criteria and Risk Assessment, Beijing 100012, China;Department of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, State Key Laboratory of Environmental Criteria and Risk Assessment, Beijing 100012, China;Department of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, State Key Laboratory of Environmental Criteria and Risk Assessment, Beijing 100012, China |
| Abstract: | |
| Keywords: | PCR-DGGE bacterial diversity nitrite-nitrification and electrochemical bio-denitrification denitrification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《环境工程学报》浏览原始摘要信息 | |
| 点击此处可从《环境工程学报》下载免费的PDF全文 | |